| 李红芬,汤由之,张智弘,张炜明,李 俊.经肺炎衣原体感染的SD大鼠的病理学特征变化分析研究[J].,2020,(22):4270-4273 |
| 经肺炎衣原体感染的SD大鼠的病理学特征变化分析研究 |
| Study on Pathological Features of SD Rats Infected by Chlamydia Pneumoniae |
| 投稿时间：2020-06-05 修订日期：2020-06-30 |
| DOI：10.13241/j.cnki.pmb.2020.22.015 |
| 中文关键词: 大鼠 肺炎衣原体 肺组织病理 炎症细胞 中性粒细胞 |
| 英文关键词: Rats Chlamydia pneumoniae Lung histopathology Inflammatory cells Neutrophils |
| 基金项目:国家自然科学基金青年基金项目（81702614） |
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| 中文摘要: |
| 摘要 目的：探讨与分析大鼠肺炎衣原体感染后的病理学特征变化。方法：研究时间为2019年5月到2020年2月。将30只斯泼累格?多雷(Sprague Dawley，SD)大鼠随机分为2组-实验组与对照组，每组各15只大鼠。实验组大鼠从鼻腔吸入40 μL 含1×103感染颗粒的肺炎衣原体，对照组吸入等剂量的无菌磷酸液缓冲液。观察与检测大鼠一般行为、血液学指标与病理学变化情况。结果：实验组肺实变面积达25 %~50 %，细支气管和小血管周围出现小灶性淋巴细胞及单个核细胞聚集，肺泡腔有大量炎性渗出，肺泡壁伴随有充血，支气管周围见大量嗜中性粒细胞浸润。小鼠一般行为表现为活力下降，毛发皱乱，进食和饮水减少，进食明显减少。接种后3 d，实验组的白细胞总数、中性粒细胞比例高于对照组，淋巴细胞比例低于对照组(P<0.05)；实验组的血清白细胞介素-6(Interleukin-6，IL-6)、肿瘤坏死因子-α(Tumor necrosis factor-α，TNF-α)浓度都显著高于对照组(P<0.05)；实验组的肺炎衣原体IgG抗体相对表达水平显著高于对照组(P<0.05)；实验组的血清血管内皮生长因子(Vascular endothelial growth factor，VEGF)、D-二聚体(D-dimer，D-D)表达水平都显著高于对照组(P<0.05)。结论：大鼠肺炎衣原体感染后伴随有肺组织病理损伤，也可诱发VEFG与D-D的表达，促进肺组织炎症细胞浸润，可导致大鼠白细胞总数、中性粒细胞比例增加，促进炎症因子的释放。 |
| 英文摘要: |
| ABSTRACT Objective: To explore and analyze the pathological characteristics of rats after Chlamydia pneumoniae infection. Methods: The study period were from May 2019 to February 2020. 30 Sprague Dawley (SD) rats were randomly divided into experimental group(n=15) and control group (n=15). The rats in the experimental group were inhaled 40 μL Chlamydia pneumoniae containing 1×103 infectious units per mL from the nasal cavity, and the control group were inhaled equal dose of sterile phosphate buffer solution. The general behavior, hematological parameters and pathological changes of the rats were observed and detected. Results: In the experimental group, the lung consolidation area WERE reached 25 % ~50 %, and there were small focal lymphocytes and mononuclear cells agglomerated around the bronchioles and small blood vessels, large amount of inflammatory exudation in the alveolar cavity, and congestion accompanied by alveolar walls. The general behavior of the mice manifested themselves as a decline in vitality, hair crumpled, eating and drinking reduced, and eating significantly reduced. 3 days after inoculation, the total number of white blood cells and the proportion of neutrophils in the experimental group were higher than those in the control group, and the proportion of lymphocytes were lower than that in the control group (P<0.05). The concentrations of IL-6, TNF-α were significantly higher than those in the control group (P<0.05), the relative expression levels of IgG antibodies to Chlamydia pneumoniae in the experimental group were significantly higher than those in the control group(P<0.05). The serum VEGF, D-D expression levels in the experimental group were significantly higher than those in the control group(P<0.05). Conclusion: After chlamydia pneumoniae infection, the rats are accompanied by pathological damage to the lung tissue and are promoted infiltration of inflammatory cells in the lung tissue, which can lead to an increase in the total number of white blood cells and the proportion of neutrophils in the rats so as to induce the expression of VEFG and D-D and promote the release of inflammatory factors. |
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