| 张 萌,张志勇,张 蕊,张晋霞,刘 斌,李世英,王雅楠,张彩凤,任 伯,曹福源,贺永贵.白藜芦醇后处理对大鼠脑缺血再灌注损伤海马CA1区Bax、Bcl-2的影响[J].,2020,(19):3644-3648 |
| 白藜芦醇后处理对大鼠脑缺血再灌注损伤海马CA1区Bax、Bcl-2的影响 |
| Effects of Resveratrol Post-processing on the Bax and Bcl-2 in Hippocampal CA1 Expressions of Cerebral Ischemia Reperfusion Rats |
| 投稿时间：2020-02-27 修订日期：2020-03-23 |
| DOI：10.13241/j.cnki.pmb.2020.19.008 |
| 中文关键词: 白藜芦醇 脑再灌注损伤 后处理 Bax Bcl-2 |
| 英文关键词: Resveratrol Cerebral ischemia reperfusion Post-processing Bax Bcl-2 |
| 基金项目:河北省教育厅科研基金项目（QN2017119）；2017年政府资助省级临床医学优秀人才课题（白藜芦醇对脑缺血再灌注大鼠的保护作用及机制研究）；河北省中医药管理局项目（2017205）；华北理工大学青年科学研究基金项目（Z201605） |
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| 中文摘要: |
| 摘要 目的：探讨白藜芦醇后处理对大鼠脑缺血再灌注损伤Bax、Bcl-2表达的影响。方法：清洁级雄性SD大鼠60只随机分为假手术组（n=12）、I/R组（n=12）、白藜芦醇组（n=36），白藜芦醇组按不同剂量分为低剂量、中剂量、高剂量组（10 mg/kg、20 mg/kg、40 mg/kg），每组12只。假手术组：仅暴露大鼠颈外动脉，不做缺血处理；I/R组：采用改良线栓法制备大鼠大脑中动脉缺血再灌注损伤模型（缺血2 h，再灌注24 h）；白藜芦醇组：造模方法同I/R组，在大鼠缺血2h后，将不同剂量白藜芦醇腹腔注射入大鼠体内，比较各组SD大鼠神经功能缺损评分、采用Western blotting法、免疫组化法对大鼠脑组织缺血侧海马CA1区Bax和Bcl-2表达进行比较。结果：白藜芦醇低、中、高剂量组神经功能缺损评分均低于I/R组，随着白藜芦醇剂量的增加，神经功能缺损评分逐渐降低，其中白藜芦醇高剂量组神经功能缺损评分降低最为明显；白藜芦醇组与I/R组相比，不同剂量白藜芦醇组Bax表达逐渐减少，而Bcl-2表达明显增加，其中以白藜芦醇高剂量组改变最为明显。结论：高剂量白藜芦醇可以降低大鼠神经功能缺损评分，减轻脑缺血再灌注损伤，对大鼠脑缺血再灌注损伤具有保护作用，其机制与Bax、Bcl-2的表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of resveratrol post-treatment on Bax and Bcl-2 expression in rats with cerebral ischemia reperfusion injury. Methods: Sixty clean grade male SD rats were randomly divided into sham surgery group (n=12), I/R model group (n=12) and resveratrol group (n=36). Resveratrol group was divided into low dose, medium dose and high dose groups (10 mg/kg, 20 mg/kg, 40 mg/kg) according to different doses, with 12 rats in each group.In sham operation group, only external carotid artery was exposed and no ischemic treatment was performed. In the I/R model group, rat middle cerebral artery ischemia-reperfusion injury model (ischemia for 2 h, reperfusion for 24 h) was prepared by the improved line plug method. Resveratrol group: modeling method was the same as I/R model group. After 2h of ischemia, different doses of resveratrol were intraperitoneally injected into rats to compare the score of nerve function defects in SD rats in each group. Western blotting and immunohistochemical methods were used to compare the expressions of Bax and Bcl-2 in the hippocampal CA1 area of ischemic brain tissue in rats. Results: The scores of neurological deficits in the low, medium and high dose groups were all lower than those in the I/R model group. With the increase of resveratrol dose, the scores of neurological deficits decreased gradually, among which the scores of neurological deficits in the high dose group decreased most significantly. In the resveratrol group, compared with the I/R model group, Bax expression gradually decreased in the resveratrol group with different doses, while Bcl-2 expression significantly increased, with the most significant change in the resveratrol high-dose group. Conclusion: The high dose resveratrol can reduce the score of neural function defect in rats, reduce cerebral ischemia-reperfusion injury, and have protective effect on cerebral ischemia-reperfusion injury in rats. Its mechanism is related to the expression of Bax and Bcl-2. |
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