| 段晨阳,向鑫明,匡 磊,刘良明,李 涛.不同组织细胞线粒体数量及功能的休克敏感性研究[J].,2020,(19):3601-3608 |
| 不同组织细胞线粒体数量及功能的休克敏感性研究 |
| The Sensitivity of Mitochondrial Quantity and Function from Different Types of Tissue to Hemorrhagic/septic Shock Stress |
| 投稿时间：2020-06-03 修订日期：2020-06-28 |
| DOI：10.13241/j.cnki.pmb.2020.19.001 |
| 中文关键词: 失血性休克 脓毒性休克 线粒体数量 线粒体功能 休克敏感性 |
| 英文关键词: Hemorrhagic shock Septic shock Mitochondrial quantity Mitochondrial function Shock sensitivity |
| 基金项目:国家自然科学基金重点项目（81730059）；国家自然科学基金青年项目（81700429） |
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| 中文摘要: |
| 摘要 目的：探究不同组织细胞线粒体数量及功能的休克敏感性差异。方法：在整体和细胞水平模拟失血性休克和脓毒性休克模型，通过mtDNA检测、线粒体形态分析和线粒体ROS检测观察休克不同时相点肠组织（肠上皮细胞）、血管组织（血管平滑肌细胞）和心肌组织（心肌细胞）中线粒体数量和功能的变化。结果：对于失血性休克（缺氧）刺激，肠组织线粒体数量的休克敏感性明显强于血管和心肌组织（P<0.05）。肠、血管、心肌组织中线粒体数量明显增多分别开始于失血性休克后0.5小时、1小时和2小时。对于脓毒性休克（LPS）刺激，肠组织线粒体数量的休克敏感性明显弱于血管和心肌组织（P<0.05）。肠、血管、心肌组织中线粒体数量明显增多分别开始于脓毒性休克后9小时、6小时和3小时。只有高浓度长时间LPS刺激才会引起肠上皮细胞线粒体数量的明显增高。各组织细胞线粒体功能对各型休克刺激的敏感性和反应程度虽然存在差异，但都晚于线粒体数量异常的发生（P<0.05）。结论：各型休克的组织器官敏感性差异可能与不同组织细胞中线粒体的休克敏感性不同有关。线粒体数量异常增加是引起休克后线粒体损伤和细胞功能障碍的始动环节，不同组织细胞线粒体的休克敏感性差异也是影响休克组织器官损伤差异的重要原因之一。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the difference of shock sensitivity in mitochondrial quantity and function from different types of tissues. Methods: The hemorrhagic shock and septic shock models were simulated at animal and cellular levels. mtDNA detection, mitochondrial morphology observation and ROS detection were undertaken to observe mitochondrial quantity and function in intestinal, vascular as well as myocardial tissues at different times of hemorrhagic/septic shock. Results: Under hemorrhagic shock or hypoxia stimulation, the sensitivity of mitochondrial quantity in intestinal tissue was significantly higher than that in vascular and myocardial tissues (P<0.05). The number of mitochondria in intestine, vascular and heart increased significantly from 0.5 h, 1 h and 2 h after hemorrhagic shock, respectively. Under septic shock or LPS stimulation, the sensitivity of mitochondrial quantity in intestinal tissue was much lower than that in vascular and myocardial tissues (P<0.05). The number of mitochondria in intestine, vascular and heart increased significantly from 9 h, 6 h and 3 h after septic shock, respectively. Only high concentration of LPS stimulation for a long time may increase the number of mitochondria in intestinal epithelial cells obviously. There were differences in sensitivity and response degree of mitochondrial function from different types of tissue to various types of shock stimulation, but they were all later than the increase in mitochondrial quantity. Conclusion: The different tissue sensitivity to various types of shock may be related to the different shock sensitivity of mitochondria in different types of tissue. The abnormal increase of mitochondrial number is the initial link of mitochondrial damage and cell dysfunctions after shock. The different shock sensitivity of mitochondria in different tissues is also one of the important causes of different injury degrees of various tissues after shock. |
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