| 潘晓南,斯依提·阿木提,刘文娟,阿地力江·伊明,童卓云,侯鹏程,努尔比亚·阿力甫.菠菜和芫荽对雄性大鼠性功能的影响与植物雌激素样作用研究[J].,2020,(18):3424-3429 |
| 菠菜和芫荽对雄性大鼠性功能的影响与植物雌激素样作用研究 |
| Effects of Spinach and Coriander on Sexual Function of Male Rats and Their Estrogen-like Effects |
| 投稿时间：2020-02-28 修订日期：2020-04-15 |
| DOI：10.13241/j.cnki.pmb.2020.18.005 |
| 中文关键词: 菠菜 芫荽 植物雌激素 性功能 雌激素受体 |
| 英文关键词: Spinach Coriander Phytoestrogens Sexual function Estrogen receptor |
| 基金项目:新疆维吾尔自治区重点研发计划项目（2016B03044-6） |
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| 中文摘要: |
| 摘要 目的：研究菠菜、芫荽对雄性大鼠性功能的影响，探讨植物雌激素样作用机制。方法：60只正常雄性SD大鼠，随机分为正常组（N组）、大豆对照组（A组）、菠菜组（B组）、芫荽组（C组）和菠菜+芫荽组（D组），每组12只，不同饲料干预20周后，进行性行为学试验并取材。采用放免法检测各组外周血清中T、E2、LH、FSH和PRL等性腺轴激素含量，HE染色观察睾丸组织形态学变化，IHC和western blot法检测大鼠睾丸中ERα，ERβ蛋白表达。结果：（1）性行为学检测结果：C和D组插入、射精潜伏期较N组显著延长（P＜0.05），爬背、插入，射精次数较N组显著减少（P＜0.05）；D组插入潜伏期较A组显著延长（P＜0.05）（2）性激素水平检测结果：D组T水平较N组显著降低（P＜0.05）；C组E2水平较A组显著升高（P＜0.05），D组E2水平较N、A组显著升高（P＜0.05）；C和D组LH水平较N组显著降低（P＜0.05），同时C组LH水平亦较A组显著降低（P＜0.05）；FSH水平在各组之间无显著差异（P＞0.05）；D组PRL水平较N组显著降低（P＜0.05）。（3）睾丸HE染色结果：光镜下N组睾丸组织结构完整，B、C和D组睾丸组织形态结构发生明显变化，生精小管间连接疏松，间质细胞减少，生精细胞减少。（4）ERα和ERβ的表达结果：ERα和ERβ分别在睾丸中精原细胞和间质细胞中表达，其中ERα在B、C和D组表达较N组显著增多（P＜0.05），ERβ在各组间无显著差异（P＞0.05）。结论：芫荽及菠菜和芫荽联合干预可延长大鼠爬背、插入、射精潜伏期和减少其爬背、插入和射精次数，导致大鼠性功能障碍；具体机制可能与发挥雌激素样作用，升高血清中E2水平，使ERα在睾丸间质中的表达增多，进而降低T水平有关。芫荽与大豆相比具有更强的雌激素样作用，而菠菜与大豆雌激素样作用接近，菠菜和芫荽联合干预出现累加效应。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects of spinach and coriander on sexual function in male rats and explore their estrogen-like effects. Methods: Sixty normal male Sprague-Dawley rats were randomly divided into normal group (N group), soy control group (A group), spinach group (B group), coriander group (C group) and spinach + coriander group (D group), 12 rats in each group. Collect samples after being conducted sexual behavior tests. The levels of T, E2, LH, FSH and PRL in the peripheral blood of each group were detected by radioimmunoassay. Observe morphology of testicular tissue by HE staining. The expressions of ERα and ERβ in rat testis were detected by IHC and western blot. Results: (1) Sexual behavior test results: the incubation periods of insertion and ejaculation of rats in groups C and D were significantly increased compared with group N(P<0.05), the frequency of straddle, insertion and ejaculation were significantly decreased compared with group N(P<0.05). The incubation periods of insertion in group D were significantly increased compared with group A (P<0.05). (2) Results of sex hormone levels: The level of T in group D was significantly lower compared with group N (P<0.05). The level of E2 in group C was significantly higher compared with group A (P<0.05). The level of E2 in group D was significantly higher compared with groups N and A. The levels of LH in groups C and D were significantly lower compared with group N (P<0.05), and LH level in group C was significantly lower compared with group A (P<0.05). The levels of FSH were not significantly different among groups (P>0.05). The PRL level in group D was significantly lower compared with group N (P<0.05). (3) HE staining results: The testicular tissue structure in group N was completed under light microscope. In groups B, C, and D, the morphological structure of testicular tissue significantly changed, the number of leydig cells and spermatogenic cells in testicular tissue reduced, and the arrangement of seminiferous tubules were loosely.(4) The results of ERα and ERβ expression: ERα and ERβ were expressed in spermatogonia and stromal cells respectively in testis. The expression of ERα in groups B, C and D were significantly increased compared with group N (P<0.05). There was no significant difference in the expression level of ERβ among groups (P>0.05). Conclusion: Coriander and spinach combined with coriander could cause sexual dysfunction in rats by prolonging the incubation periods of straddle, insertion and ejaculation and reducing the frequency of straddle, insertion and ejaculation. The specific mechanism may be related to decreased T level through exerting estrogen-like effects, increasing serum E2 level and increasing the expression of ERα in Leydig cells. Coriander has a stronger estrogen-like effects than soy. Spinach and soy have similar estrogen-like effects. The combined feed of spinach and coriander has a cumulative effect. |
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