| 刘含若,白玮玲,夏子尧,董 喆,宋旭东.lncRNA BANCR 在晶体上皮细胞上皮-间质转化，增殖、凋亡及自噬的作用[J].,2020,(17):3243-3247 |
| lncRNA BANCR 在晶体上皮细胞上皮-间质转化，增殖、凋亡及自噬的作用 |
| The Effect of lncRNA BANCR on EMT, Proliferation, Apoptosis and Autophagy in Human Lens Epithelial Cells |
| 投稿时间：2020-05-06 修订日期：2020-05-30 |
| DOI：10.13241/j.cnki.pmb.2020.17.009 |
| 中文关键词: 后发性白内障，长链非编码RNA BANCR，自噬 |
| 英文关键词: After cataract Long non-coding RNA BANCR Autophagy |
| 基金项目:国家自然科学基金项目（81700813）；北京市医院管理局"青苗"计划专项经费（QML20180205）；北京市优秀人才培养资助项目；北京市科技新星项目（Z191100001119072）；首都医科大学附属北京同仁医院拔尖人才培养计划，医药协同科研创新研究专项（Z181100001918035） |
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| 中文摘要: |
| 摘要 目的：探索长链非编码RNA BANCR（lncRNA BANCR） 在人晶状体上皮细胞FHL24中对上皮-间质转化的作用，并进一步探究了其调控晶体上皮细胞增殖、凋亡及自噬的作用及相关分子机制。方法：运用qReal - time PCR 检测TGF-β诱导对FHL24细胞内EMT相关标志物α-SMA，E-cadherin，Coll I ，ZO1及BANCR mRNA相对表达量。细胞中转染BANCR。Western 印迹检测各组细胞中EMT相关标志蛋白及LC3Ⅱ/ Ⅰ的蛋白表达。MTT 法检测各组细胞的增殖，凋亡情况。结果：与正常对照组比较，TGF-β诱导组细胞中BANCR, α-SMA, Coll I， ZO1 mRNA的相对表达量明显增加, 而E-cadherin mRNA显著下降, 差异均有统计学意义 (t=-5.031, -7.145, -9.023, -6.012, 5.097均P<0.05)，以上因子的蛋白表达趋势相同，差异均有统计学意义（均P<0.05)。siRNA-BANCR TGF-β诱导组细胞中E-cadherin mRNA相对表达量比 siRNA TGF-β诱导组显著增加(t=-9.98, P<0.05）；α-SMA，Coll I 及ZO1 mRNA相对表则显著减少（t=9.003; 27.738; 19.620, P<0.05）。抑制BANCR后细胞增殖活力48、72 h 时细胞活性显著降低(t=5.032, 9.041，均P<0.05），细胞凋亡率显著升(t=16.772，P<0.001）。自噬标志蛋白LC3 - II/ LC3 - I 比例增加 (P <0.05)。结论：长链非编码BANCR参与了晶体上皮细胞的抑制其上皮-间质转化，抑制BANCR可抑制晶体上皮细胞增殖、增加凋亡及自噬的发生。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of long non-coding RNA BANCR (lncRNA BANCR) on epithelial-mesenchymal transition in human lens epithelial cells FHL24, and to further explore its role in regulating the proliferation, apoptosis and autophagy of lens epithelial cells and related molecular mechanisms. Methods: qReal-time PCR was used to detect the relative expression of EMT-related markers α-SMA, E-cadherin, Coll I, ZO1 and BANCR mRNA induced by TGF-β in FHL24 cells. Transfect the cells with BANCR. Western blotting was used to detect the expression of EMT-related marker proteins and LC3Ⅱ/I in each group of cells. MTT method was used to detect cell proliferation and apoptosis in each group. Results: Compared with the normal control group, the relative expression of BANCR, α-SMA, Coll I, and ZO1 mRNA in the cells of the TGF-β induction group increased significantly, while the E-cadherin mRNA decreased significantly, and the differences were statistically significant (t=-5.031, -7.145, -9.023, -6.012, 5.097 all P<0.05), the protein expression trends of the above factors were the same, and the differences were statistically significant (all P<0.05). The relative expression of E-cadherin mRNA in the cells of the siRNA-BANCR TGF-β induction group was significantly higher than that in the siRNA TGF-β induction group (t=-9.98, P<0.05); the relative expression of α-SMA, Coll I and ZO1 mRNA was significant Decrease (t=9.003; 27.738; 19.620, P<0.05). After inhibiting BANCR, the cell proliferation activity was significantly reduced at 48 and 72 h (t=5.032, 9.041, all P<0.05), and the apoptosis rate was significantly increased (t=16.772, P<0.001). The ratio of autophagy marker protein LC3-II/LC3-I increased (P<0.05). Conclusion: Long-chain non-coding BANCR is involved in the inhibition of epithelial-mesenchymal transition of lens epithelial cells. Inhibition of BANCR can inhibit the proliferation of lens epithelial cells, increase apoptosis and autophagy. |
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