| 邢天容,吕敬媛,朴英兰,佟丽波,张 燕.沙利度胺联合放疗对宫颈癌裸鼠移植瘤的VEGF、bFGF、TNF-α表达的影响[J].,2020,(16):3038-3042 |
| 沙利度胺联合放疗对宫颈癌裸鼠移植瘤的VEGF、bFGF、TNF-α表达的影响 |
| Effects of Thalidomide Combined with Radiotherapy on the VEGF, bFGF, TNF-α Expression in Cervical Cancer Nude Mice |
| 投稿时间：2020-02-03 修订日期：2020-02-27 |
| DOI：10.13241/j.cnki.pmb.2020.16.008 |
| 中文关键词: 沙利度胺 宫颈癌 移植瘤 放疗增敏 肿瘤延迟生长时间 放射增敏比 |
| 英文关键词: Thalidomide Cervical carcinoma Transplanted tumor Radiosensitization Delayed tumor growth time Radiosensitization ratio |
| 基金项目:国家卫生计生委医药卫生科技发展研究中心项目(W2015CAE113) |
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| 中文摘要: |
| 摘要 目的：分析沙利度胺联合放疗对宫颈癌裸鼠移植瘤的VEGF、bFGF、TNF-α表达的影响，探究其增敏作用的机制。方法：选择24只裸鼠，均在左侧腋部皮下注射0.2 mL Hela细胞悬浊液进行造模。将造模成功的裸鼠随机分成对照组(Control group，CG)、沙利度胺组(Thalidomide group，TG)、放疗组(Radiotherapy group，RG)和联合治疗组(TG+RG)，每组6只，分别给予羧甲基纤维素钠、沙利度胺溶液、放疗、沙利度胺溶液+放疗处理。治疗14 d后，通过检测裸鼠肿瘤体积、肿瘤抑制率、肿瘤延迟生长时间(Delayed tumor growth time，TGD)、放射增敏比(Radiosensitization ratio，SER)、肿瘤细胞的坏死程度、微血管密度、血清中血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)及肿瘤组织中VEGF、bFGF、肿瘤坏死因子-α(TNF-α)分析沙利度胺联合放疗对宫颈癌裸鼠移植瘤的放疗增敏作用及其机制。结果：治疗14 d后，与对照组相比，沙利度胺组、放疗组和联合治疗组裸鼠肿瘤体积均明显变慢，肿瘤抑制率均显著增高，TGD均显著均明显延长，坏死细胞个数均明显增多，微血管密度、血清和肿瘤组织中VEGF、bFGF、TNF-α表达量均明显降低(P＜0.05)；与沙利度胺组和放疗组相比，联合治疗组裸鼠瘤重均明显变慢，肿瘤抑制率均显著增高，TGD均显著均明显延长，坏死细胞个数均明显增多，微血管密度、血清和肿瘤组中VEGF、bFGF、TNF-α表达量均明显降低(均P＜0.05)；放射增敏比(SER)大于1。结论：沙利度胺可显著增强放疗对宫颈癌裸鼠移植瘤的效果，可能与其显著降低VEGF、bFGF、TNF-α的表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the effects of thalidomide combined with radiotherapy on the expression of the VEGF, bFGF and TNF-α in nude mice with cervical cancer xenografts, and to explore the mechanism of their sensitizing effect. Methods: Twenty-four nude mice were selected, and 0.2 mL Hela cell suspension was injected subcutaneously into the left armpit for modeling. The nude mice with successful modeling were randomly divided into the control group (CG), the thalidomide group (TG), the radiotherapy group (RG) and the combined treatment group (TG+RG), 6 per group. Each group was given carboxymethylcellulose sodium, thalidomide solution, radiotherapy, thalidomide solution+radiotherapy. After 14 days of treatment, the tumor volume, tumor inhibition rate, delayed tumor growth time (TGD) and radiosensitization were measured Ration, Ser), necrosis degree of tumor cells, microvessel density, VEGF, bFGF in serum and VEGF, bFGF, TNF-α in tumor tissue were detected. The radiosensitization effect and mechanism of thalidomide combined with radiotherapy on cervical cancer transplanted tumor in nude mice were analyzed. Results: After treatment 14 d, compared with the control group, the growth rate of tumor volume in the thalidomide group, the radiotherapy group and the combined treatment group was significantly slower, tumor inhibition rate was significantly higher, TGD was significantly longer, the number of necrotic cells was significantly increased, microvascular density, serum and the expression of VEGF, bFGF, TNF-α in tumor group were significantly reduced, the difference was statistically significant (P<0.05). Compared with the thalidomide group and the radiotherapy group, the tumor weight in the combined therapy group was significantly slower, the tumor inhibition rate was significantly higher, the TGD was significantly longer, the number of necrotic cells was significantly increased, the expression of VEGF, bFGF and TNF-α in microvessel density, serum and tumor group were significantly lower, the difference was statistically significant (P<0.05). The sensitivity ratio (SER) is greater than 1. Conclusion: Thalidomide could significantly enhance the effect of radiotherapy on cervical cancer xenografts in nude mice, which may be related to its significant reduction in the expression of VEGF, bFGF, and TNF-α. |
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