| 吴 楠,夏 阳,李曼曼,王 朕,李秋实,仰曙芬.补充维生素D对小鼠发育行为的影响研究[J].,2020,(15):2824-2829 |
| 补充维生素D对小鼠发育行为的影响研究 |
| Effects of Vitamin D Supplementation on Neurobehavior of Mice |
| 投稿时间：2020-02-21 修订日期：2020-03-18 |
| DOI：10.13241/j.cnki.pmb.2020.15.005 |
| 中文关键词: 维生素D 孕期维生素D缺乏 神经行为 |
| 英文关键词: Vitamin D Vitamin D deficiency during pregnancy Neurobehavior |
| 基金项目:黑龙江省科技攻关重点项目（GC12C306） |
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| 中文摘要: |
| 摘要 目的：本研究通过对子代小鼠维生素D的干预，探讨维生素D对子代小鼠行为的重要影响，为孕期补充维生素D提供理论依据。方法：建立孕鼠维生素D缺乏的实验模型。将子代小鼠分为三组：肌注维生素D的低剂量饮食组（a组，补充组）、肌注生理盐水的低剂量饮食组（b组，缺乏组）和正常饮食组（c组）。在第24天和第60天时，采用旷场实验、Morris水迷宫实验、社交试验对子代小鼠的行为进行监测。结果：孕期维生素D缺乏对子代小鼠的维生素D水平有显著的影响，子代小鼠出生10天时血清维生素D的浓度分别为：低剂量饮食组12.98±0.65 μg/L，正常饮食组35.38±1.13 μg/L，两组浓度值差异显著（P<0.05）。旷场实验中，第24天时，缺乏组子代小鼠的运动总路程为1044±89.21 cm，显著少于补充组（1701.56±150.5 cm）和正常组（1755±154.2 cm），而其在中央区停留的时间为34.84±3.54 s，显著高于补充组（21.36±3.05 s）和正常组（21.77±3.64 s），其在周边停留的时间为265.2±3.54 s，显著少于补充组（278.6±3.05 s）和正常组（278.2±3.64 s）；第60天时缺乏组子代小鼠在中央区停留的时间为28.79±3.68 s，显著高于补充组（17.21±2.59 s）和正常组（18.37±1.99 s），其在周边停留的时间为271.2±3.68 s，显著少于补充组（282.7±2.54 s）和正常组（281.6±1.99 s）。缺乏组在第60天时行动总路程为1653±141 cm低于其他两组，但差异不显著。Morris水迷宫实验中，三组子代小鼠第24天的穿越次数均显著高于第60天，呈现先增加后降低的趋势，而缺乏组的降低趋势更为明显。平台停留时间方面，三组子代小鼠在第60天均多于第24天，呈增加趋势。社交实验中，第24天时三组小鼠探索空笼子的时间没有明显差异，但缺乏组小鼠与代表新伙伴的陌生鼠1接触的时间低于与空笼子的接触时间，而正常组和补充组小鼠与陌生鼠接触的时间明显多于空笼子。第60天时三组小鼠探索空笼子的时间也没有明显差异，虽然缺乏组小鼠与陌生鼠的接触时间略高于与空笼子的接触时间，但差异不显著，而正常组和补充组小鼠与陌生鼠接触的时间仍明显多于空笼子。结论：向维生素D缺乏的子代小鼠补充维生素D能够对子代小鼠的兴奋性、空间认知能力、学习记忆能力和社交行为产生良性影响，在发育早期进行维生素D补充具有重要的预防作用。 |
| 英文摘要: |
| ABSTRACT Objective: Through the intervention of vitamin D in offspring mice, this study explored the important influence of vitamin D on behavior of offspring mice, and provided theoretical basis for vitamin D supplement during pregnancy. Methods: To establish an experimental model of vitamin D deficiency in pregnant mice. The offspring mice were divided into three groups: the low-dose diet group (group A, supplemented group), the low-dose diet group (group B, deficient group) and the normal diet group (Group C). On the 24th and 60th day, open field experiment, Morris water maze experiment and social interaction experiment were used to monitor the behavior of the offspring mice. Results: Vitamin D deficiency during pregnancy had a significant effect on the level of vitamin D in offspring mice. The concentration of serum vitamin D in offspring mice was 12.98±0.65 μg/L in the low dose diet group and 35.38±1.13 μg/L in the normal diet group, respectively, with a significant difference between the two groups (P<0.05). In the open field test, on the 24th day, the total movement distance of the mice in the deficient group was 1044±89.21 cm, significantly less than that in the supplement group (1701.56±150.5 cm) and the normal group (1755±154.2 cm), while the duration of staying in the central area was 34.84±3.54 s, significantly higher than that in the supplement group (21.36±3.05 s) and the normal group (21.77±3.64 s), and the duration of staying around was 265.2±3.54 s, significantly less than that in the supplement group (278.6±3.05 s) and the normal group (278.2±3.64 s) on the 60th day, the duration of stay in the central area of mice in the deficient group was 28.79±3.68 s significantly higher than that in the supplement group (17.21±2.59 s) and the normal group (18.37±1.99 s), and the duration of stay in the peripheral area was 271.2±3.68 s significantly shorter than that in the supplement group (282.7±2.54 s) and the normal group (281.6±1.99 s). The total distance of 1653±141 cm in the deficiency group was lower than that in the other two groups, but the difference was not significant. In Morris water maze test, the crossing times of the three groups of mice on the 24th day were significantly higher than those on the 60th day, showing the trend of increasing first and then decreasing, while the trend of decreasing in the deficient group was more obvious. As for platform residence time, the number of offspring mice in the three groups was more on the 60th day than on the 24th day, showing an increasing trend.In the social interaction test, there was no significant difference in the time of exploring the empty cage among the three groups on the 24th day, but the contact time between the deficient group and the strange mouse (stranger1) representing the new partner was shorter than that with the empty cage, while the contact time between the normal group and the supplement group was significantly longer than that of the empty cage. On the 60th day, there was no significant difference in the time of exploring the empty cage among the three groups. Although the contact time between the deficient group and the strange mice was slightly longer than that between the deficient group and the empty cage, the difference was not significant, while the contact time between the normal group and the supplement group was still significantly longer than that between the empty cage. Conclusion: The supplement of vitamin D to the offspring of mice with vitamin D deficiency can have a positive effect on the excitability, spatial cognitive ability, learning and memory ability and social behavior of the mice offspring, vitamin D supplementation in early development has an important preventive effect. |
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