| 范晓东,靳晓柳,卢 强,张继朋,王武平,李 哲.雌激素对A549细胞系EMT过程的影响[J].,2020,(14):2635-2639 |
| 雌激素对A549细胞系EMT过程的影响 |
| Effects of Estrogen on Epithelial Mesenchymal Transition Process of A549 Cell Line |
| 投稿时间：2020-02-26 修订日期：2020-03-22 |
| DOI：10.13241/j.cnki.pmb.2020.14.007 |
| 中文关键词: 雌激素 上皮间质转化 肺腺癌 |
| 英文关键词: Estrogen Epithelial Mesenchymal Transition Lung adenocarcinoma |
| 基金项目:国家自然科学基金项目（81572252） |
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| 中文摘要: |
| 摘要 目的：探讨雌激素对A549细胞系EMT标志物表达量的影响。方法：用不同浓度的雌激素刺激A549细胞系，并用q-RT-PCR和Western-blot实验检测各组细胞中EMT标志物表达量的变化，用Transwell实验检测不同浓度雌激素对细胞迁移能力的影响，计算各组间有无统计学差异。结果：当雌激素浓度为1×10-9 mol/L、1×10-8 mol/L、1×10-7 mol/L时，Vimentin的mRNA表达量分别为：2.14±0.55、4.72±0.63、2.21±0.47，显著高于空白对照组，组间有统计学差异，E-cadherin的mRNA表达量分别为：0.64±0.15、0.46±0.11、0.59±0.13，显著低于空白对照组，组间有统计学差异，蛋白表达量也有同样趋势；细胞迁移数分别为58.63±7.33、80.12±9.32、61.89±8.22，组间有统计学差异。当雌激素浓度为1×10-8 mol/L时，Vimentin的表达量最高，E-cadherin的表达量最低，细胞迁移数最高。结论：适宜浓度雌激素可以促进Vimentin的表达，抑制E-cadherin的表达，提高细胞迁移能力，当雌激素浓度为1×10-8 mol/L时，促进Vimentin表达、抑制E-cadherin表达和促进细胞迁移的作用最显著。由此认为，雌激素对A549细胞系发生EMT过程有促进作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of estrogen on the EMT process of A549 cell line. Methods: The A549 cell line was stimulated with different concentrations of estrogen, and the expression of EMT markers in each group was detected by q-RT-PCR and Western blot. The effect of different concentrations of estrogen on cell migration was detected by Transwell experiment, and the statistical difference between each group was calculated. Results: When the concentration of estrogen was 1×10-9 mol/L, 1×10-8 mol/L, 1×10-7 mol/L, the mRNA expression of vimentin was 2.14±0.55, 4.72±0.63 and 2.21±0.47, which was significantly higher than that of the blank control group, the mRNA expression of E-cadherin was 0.64±0.15, 0.46±0.11 and 0.59±0.13, which was significantly lower than that of the blank control group, the protein expression had the same trend as mRNA, the number of cell migration was 58.63 ±7.33, 80.12±9.32, 61.89±8.22, which was significantly higher than that of the blank control group. When the concentration of estrogen was 1×10-8 mol/L, the expression of vimentin was the highest, the expression of E-cadherin was the lowest, and the number of cell migration was the highest. Conclusion: The appropriate concentration of estrogen can promote the expression of vimentin, inhibit the expression of E-cadherin, and improve the ability of cell migration. When the concentration of estrogen is 1×10-8 mol/L, the effect of promoting the expression of vimentin, inhibiting the expression of E-cadherin and promoting cell migration is the most significant. Therefore, estrogen can promote the EMT process of A549 cell line. |
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