| 刘 畅,李海峰,陈点点,阮新建,刘彦芳.红色诺卡氏菌细胞壁骨架对小鼠肝癌细胞生长的抑制效果[J].,2020,(14):2614-2617 |
| 红色诺卡氏菌细胞壁骨架对小鼠肝癌细胞生长的抑制效果 |
| The Effect of N-CWS on Tumor Inhibition of H22 BALB/c Mice |
| 投稿时间：2020-02-02 修订日期：2020-02-27 |
| DOI：10.13241/j.cnki.pmb.2020.14.003 |
| 中文关键词: 诺卡氏菌 免疫增强剂 荷瘤小鼠 肿瘤免疫 |
| 英文关键词: Nocardia rubrum Biological Response Modifiers Tumor BALB/c mice Tumor immunity |
| 基金项目:国家自然科学基金项目（81073087） |
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| 中文摘要: |
| 摘要 目的：通过构建H22荷瘤小鼠模型，观察红色诺卡氏菌细胞壁骨架(Nocardia Rubra Cell Wall Skeleton，N- CWS) 对H22荷瘤小鼠肝癌细胞生长的抑制效果。方法：复苏并培养H22细胞，将H22细胞以皮下方式接种到小鼠右侧腋部，建立H22荷瘤小鼠肝癌模型，随机分为对照组（生理盐水组）、低浓度N-CWS组（400 μg/mL）与高浓度N-CWS组（800 μg/mL），分别灌胃1次/d，共灌胃14 d，最长接种肿瘤15 d后处死小鼠，观察肝癌细胞的大小和重量。结果：治疗组小鼠肿瘤质量明显低于对照组（1.46±0.16）g，且高浓度组小鼠肿瘤质量（0.52±0.13）g明显低于低浓度组（0.79±0.13）g，组间对比差异有统计学意义（P<0.05）。在治疗第3 d起直至结束，治疗组小鼠肿瘤体积均明显低于对照组，从第5 d起直至结束，高浓度组小鼠肿瘤体积明显低于低浓度组（P<0.05）。第5 d起至结束，治疗组小鼠肿瘤体积增长的变化明显缓于对照组（P＜0.05）。高浓度组小鼠肿瘤体积的增长在第5 d后明显缓于低浓度组（P＜0.05）。对照组在第9 d时，增长变化最为显著，较第7 d增加约400 mm3。低浓度组变化较稳定。高浓度组肿瘤增长明显变缓，第7 d后最为明显，后逐渐趋于缓和。结论：N-CWS可以明显抑制肿瘤生长，且高浓度N-CWS抑制肿瘤生长效果更佳。 |
| 英文摘要: |
| ABSTRACT Objective: By constructing of H22 tumor-bearing mouse model, observed the inhibiting effect of N-CWS on the tumor growth of H22 tumor-bearing mice. Methods: H22 cells were recovered and cultivated, and were subcutaneously inoculated into the right armpit of the mice. The models of H22 tumor BALB/c mice were established and randomly divided into three groups, the control group (normal saline group), the low concentrationN-CWS group (400 μg/mL) and the high concentrationN-CWS group (800 μg/mL). The three groups oral administration for 14 d, once a day, and mice were sacrificed after the longest tumor inoculation for 15 days. The size and weight of liver cancer cells were observed. Results: The tumor mass of mice in the treatment group was significantly lower than that in the control group (1.46±0.16) g, and the tumor mass of mice in the high concentration group (0.52±0.13) g was significantly lower than that in the low concentration group (0.79±0.13) g. The difference between the groups was statistically significant (P<0.05). From the 3rd day to the end of the treatment, the tumor volume of the mice in the treatment group was significantly lower than that of the control group. From the 5th day to the end, the tumor volume of the mice in the high concentration group was significantly lower than that in the low concentration group (P<0.05). From the 5th day to the end, the change of tumor volume growth in the treatment group was significantly slower than that in the control group (P<0.05). The tumor volume growth of mice in high concentration group was significantly slower than that in low concentration group after 5 days (P<0.05). The control group had the most significant change on the 9th day, with an increase of about 400 mm3 compared with the 7th day. The change in the low concentration group was more stable. The tumor growth in the high-concentration group was significantly slowed down, the most obvious after 7 days, and then gradually eased. Conclusion: N-CWS can significantly inhibit tumor growth, and high concentration of N-CWS has a better effect on tumor growth. |
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