| 林 慧,陈 钱,单秋生,郭净洁,王 崇,李国林.微波消融对骨肉瘤肺转移的影响[J].,2020,(11):2054-2058 |
| 微波消融对骨肉瘤肺转移的影响 |
| Effect of Microwave Ablation on the Lung Metastasis of Osteosarcoma |
| 投稿时间：2019-12-23 修订日期：2020-01-18 |
| DOI：10.13241/j.cnki.pmb.2020.11.010 |
| 中文关键词: 微波消融 骨肉瘤 肺转移 |
| 英文关键词: Microwave ablation Osteosarcoma Metastasis Animal models |
| 基金项目:国家自然科学基金项目(81272466)；黑龙江省自然科学基金项目(H2018041) |
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| 中文摘要: |
| 摘要 目的：探讨微波消融治疗骨肉瘤对其肺转移的影响及可能机制。方法：用含有1×107个大鼠源骨肉瘤UMR106细胞的悬液于SD大鼠右下肢皮下注射建立骨肉瘤移植模型，将其随机分为对照组和消融组。消融组大鼠肿瘤予以微波消融治疗10 min，50 ℃，空白对照组大鼠模拟消融10 min，0 ℃，检测和比较两组大鼠肿瘤直径变化，于治疗15天后处死大鼠，取大鼠肿瘤及肺组织，通过HE染色以及免疫组化检测基质金属蛋白酶(Matrix metalloproteinase，MMPs)及其拮抗因子(Tissue inhibitors metalloproteinase)，即MMP-2、MMP-9及TIMP-2的表达。结果：消融组大鼠肿瘤直径明显小于对照组大鼠肿瘤直径，消融组大鼠肿瘤组织及肺组织MMP-2及MMP-9的阳性表达均显著高于对照组(P<0.05)，而TIPM-2的阳性表达则明显低于对照组(P<0.05)。结论：微波消融技术治疗骨肉瘤可以抑制骨肉瘤细胞的增殖，甚至引起细胞凋亡，并且通过上调MMP-2、MMP-9以及下调TIMP-2的表达，可能会提高其远处转移发生的风险。 |
| 英文摘要: |
| ABSTRACT Objective: In this study, we discuss the effect of microwave ablation on the lung metastasis of osteosarcoma and its mechanism of action. Methods:: Animal models of osteosarcoma transplantation were established by subcutaneous injection of 1×107 rat derived osteosarcoma UMR106 cells into the right lower limb of SD rats , which was divided them into control group and ablation group randomly. The microwave ablation ablation group rats would be treated by microwave ablation ablation for 10 min, 50 ℃, and the control group rats would be simulated ablation 10 min, 0 ℃, record the tumor diameter changes, two groups of rats would be executed after 15 days. Lung tissue was taken for HE staining. Detect the relative expression level of MMP-2 and MMP-9 and TIMP-2 in the tumor and lung tissues by immunohistochemistry. Results: The tumor diameter of the ablation group was significantly smaller than that of the blank group.The positive expression of MMP-2 and MMP-9 of tumor and lung tissues in the ablation group were significantly higher than that in the control group(P<0.05), while the positive expression of TIMP-2 was significantly lower than that in the control groups(P<0.05). Conclusion: Microwave ablation can inhibit the proliferation of osteosarcoma cells and even cause apoptosis. Because of up-regulation of the expression of MMP-2, MMP-9 and reduce TIMP-2 expression, Microwave ablation may increase the possibility of lung metastasis. |
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