| 韩思思,孙春斌,刘飞羽,韩妮萍,邢海晶,袁嘉丽.加味参苓白术散对COPD大鼠血清IL-8和TNF-α的影响[J].,2020,(10):1846-1849 |
| 加味参苓白术散对COPD大鼠血清IL-8和TNF-α的影响 |
| Effect of Modified Shenling Baizhu Powder on Serum IL-8 and TNF-α in COPD Rats |
| 投稿时间：2019-09-23 修订日期：2019-10-18 |
| DOI：10.13241/j.cnki.pmb.2020.10.010 |
| 中文关键词: 慢性阻塞性肺疾病 加味参苓白术散 炎性因子 肺组织病理 |
| 英文关键词: COPD Modified Shenling Baizhu Powder Inflammatory factor Pulmonary histopathology |
| 基金项目:云南省高校中医药调节机体微环境应用基础研究科技创新团队（2018HC011）；云南省科学技术厅应用基础研究联合专项[2018FF001(-001）] |
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| 中文摘要: |
| 摘要 目的：观察加味参苓白术散对COPD肺脾气虚证大鼠血清IL-8和TNF-α的影响。方法：将大鼠随机分为空白组、模型组及加味参苓白术散低、中、高剂量组。除空白组外，其余四组采用气管滴注脂多糖加烟熏28天的方法复制COPD模型，大黄水煎液灌胃8天的方法复制肺脾气虚证的模型，药物干预组给予加味参苓白术散治疗。HE染色观察肺组织病理情况，酶联免疫吸附法(ELISA)检测血清中IL-8、TNF-α的表达情况。结果：药物干预组大鼠炎性细胞浸润以及肺大泡融合等症状相较于模型组明显缓解，血清IL-8、TNF-α的表达降低。结论：加味参苓白术散能够改善COPD肺脾气虚证大鼠肺组织病理损伤，降低血清炎症因子IL-8、TNF-α的表达，改善炎症。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the effect of Modified Shenling Baizhu Powder on lung tissue, serum IL-8 and TNF-α in chronic obstructive pulmonary disease (COPD)lung-spleen-deficiency rats. Methods: The rats were randomly divided into blank group, model group and low, medium and high dose group. Except the blank group, the rest four groups used tracheal drip lipopolysaccharide and smoke for 28 days to replicate the COPD model, rhubarb decoction was administered for 8 days to replicate the model of lung-spleen deficiency syndrome. The drug intervention group was treated with Modified Shenling Baizhu Powder. HE staining was used to observe the pathological conditions of lung tissues. The expression of IL-8 and TNF-α in serum was detected by ELISA. Results: Compared with the model group, the symptoms of inflammatory cell infiltration and pulmonary bullae fusion in the drug intervention group were significantly relieved, and the expression of serum IL-8 and TNF-α was decreased. Conclusion: Modified Shenling Baizhu Powder can improve the pathological injury of lung tissue in COPD rats with lung-spleen deficiency syndrome, reduce the expression of serum inflammatory cytokines IL-8 and TNF-α, and improve the inflammatory. |
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