| 殳 洁,孙寒晓,张 洁,李 月,盛慧明.1型糖尿病小鼠模型中MZB和FoB细胞的检测[J].,2020,(10):1815-1819 |
| 1型糖尿病小鼠模型中MZB和FoB细胞的检测 |
| Detection of B Cell Subsets in Type 1 Diabetic Mice |
| 投稿时间：2019-10-28 修订日期：2019-11-23 |
| DOI：10.13241/j.cnki.pmb.2020.10.004 |
| 中文关键词: 1型糖尿病 链脲佐菌素 边缘区B细胞 滤泡状B细胞 |
| 英文关键词: Type 1 diabetes Streptozotocin Marginal zone B cell Folicular B cell |
| 基金项目:国家自然科学基金项目（81672083，81702071）；上海市科委自然科学基金项目（15ZR1437900） |
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| 中文摘要: |
| 摘要 目的：探究脾脏边缘区B细胞（marginal zone B cell，MZB）和滤泡状B细胞（folicular B cell，FoB）在链脲佐菌素（streptozocin，STZ）诱导的1型糖尿病小鼠模型中的频率变化。方法：以C57BL/6小鼠为研究模型，腹腔注射STZ 55 mg/kg，连续注射5天，建立1型糖尿病模小鼠模型。选取随机血糖水平≥200 mg/dL(11.1 mmol/L) 的小鼠视为造模成功。期间观察并记录小鼠摄食、饮水情况，监测小鼠体重、空腹血糖情况。造模第4周处死小鼠，随后测定小鼠的糖化血红蛋白(glycated hemoglobin, HbA1c)、谷氨酸脱羧酶抗体(GAD65)、胰岛素自身抗体(IAA)水平。通过对各组小鼠胰腺进行苏木精-伊红 (hematoxylin-eosin, H&E) 染色、免疫组化(immunohistochemistry，IHC)观察胰岛形态变化及胰岛素含量。通过流式细胞术，比较各组小鼠脾脏中MZB和FoB细胞的频数和表型。结果：与正常对照组小鼠比较，STZ糖尿病模型组小鼠摄食量、饮水量明显增加，体重减轻，随机血糖显著升高，同时HbA1c 水平明显增加(P＜0.0001)，模型组小鼠血浆GAD65和IAA水平也明显升高。H&E、IHC结果显示，与正常对照小鼠比较，STZ糖尿病小鼠胰岛萎缩、呈不规则；胰岛素颗粒数减少。与正常对照组小鼠相比，模型组小鼠脾脏MZB细胞频数增加，差异有统计学意义(P<0.05)；与正常对照组小鼠相比，模型组小鼠脾脏FoB细胞频数无统计学差异。结论：STZ诱导的1型糖尿病小鼠脾脏中MZB细胞频数升高，FoB细胞频数变化无统计学差异。这些B 淋巴细胞亚群频率失衡可能与1型糖尿病的发生有关。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the frequency change of marginal zone B cell (MZB) and folicular B cell (FoB) in streptozocin-induced type 1 diabetes mouse model. Methods: C57BL/6 mice were used as the research model, and STZ 55mg/kg was intraperitoneally injected for 5 consecutive days to establish the type 1 diabetes model mouse model. Mice with random blood glucose level ≥ 200 mg/dL (11.1 mmol/L) were considered as successful model. During this time, diet and drinking water of the mice as well as the body weight and fasting blood glucose of the mice were monitored. Four weeks later from the last injection, mice were sacrificed and then Hemoglobin(HbA1c), glutamic acid decarboxylase(GAD65) antibody and insulin autoantibody (IAA) were measured. Hematoxylin-eosin (H&E) staining and immunohistochemistry(immunohistochemistry，IHC) showed the islet morphology change and insulin content. The changes in frequency and phenotype of MZB and FoB in spleen of each group were detected by flow cytometry. Results: Compared with the normal control group, mice of the STZ diabetes model group showed significantly increased food and water intake but body weight, at the same time fasting blood glucose and glucose HbA1c (P<0.0001) as well as plasma GAD65 and IAA levels in the model group were also significantly increased. H&E and IHC showed that the islets of STZ diabetic mice were atrophic and irregular compared with normal control mice, meanwhile the number of insulin particles decreased. Compared with the control group, the frequency of MZB cells in the spleen of the model group significantly increased (P<0.05), and there was no statistical difference in the frequency of FoB cells in the spleen of the model group. Conclusion: The frequency of MZB cells increased in spleen of type 1 diabetic mice induced by STZ, and there was no statistical difference in the frequency of FoB cells. The frequency imbalance of these B lymphocyte subsets may be related to the development of type 1 diabetes. |
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