| 刘 静,何蓉蓉,余云华,张冬梅.老年胃癌相关基因的生物信息学差异表达基因初步分析[J].,2020,(7):1381-1384 |
| 老年胃癌相关基因的生物信息学差异表达基因初步分析 |
| Preliminary Analysis of Differentially Expressed Genes in Bioinformatics of Elderly Gastric Cancer Related Genes |
| 投稿时间：2019-09-02 修订日期：2019-09-29 |
| DOI：10.13241/j.cnki.pmb.2020.07.041 |
| 中文关键词: 老年胃癌 差异表达基因 生物信息学分析 |
| 英文关键词: Gastric cancer in the elderly DEGs Bioinformatics analysis |
| 基金项目:福建省自然科学基金项目（2018J01354） |
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| 中文摘要: |
| 摘要 目的：通过生物信息学方法分析老年胃癌与正常癌旁组织间的差异表达基因（Differentially expressed genes，DEGs）。方法：从癌症基因组图谱（The Cancer Genome Atlas，TCGA）数据库下载基因表达谱，使用R软件筛选DEGs，通过STRING数据库构建DEGs蛋白互作网络，采用Cytoscape软件筛选网络中关键模块，运用FunRich软件对关键模块基因进行富集分析，并且对网络中的基因进行总生存期分析。结果：共筛选出163个DEGs，富集结果显示：关键模块基因的分子功能主要富集在MHC受体活性；生物过程主要富集在免疫反应；生物通路富集在Notch信号通路；生存分析发现高表达的SP8转录因子(SP8 transcription factor, SP8)基因导致老年胃癌患者总生存率降低。结论：通过本研究生物信息学方法分析的老年胃癌所富集的信号通路和SP8基因可能在老年胃癌的发生中具有作用。 |
| 英文摘要: |
| ABSTRACT Objective: To analyse the differentially expressed genes (DEGs) between elderly gastric cancer and normal adjacent tissues by bioinformatics methods, and to explore the pathogenesis of gastric cancer in the elderly at the molecular level, and to provide new ideas for its diagnosis and prognosis. Methods: Download gene expression profiles from the Cancer Genome Atlas (TCGA) database and screen DEGs using R software, construction of DEGs protein-protein interaction network through STRING database, using Cytoscape software to screen the key module in the network, enrichment analysis of key module genes using FunRich software and the overall survival analysis of the genes in the network is performed. Results: A total of 163 DEGs were screened out. The enrichment results showed that molecular function of key module genes was mainly enriched in MHC receptor activity; Biological process was mainly enriched in the immune response; Biological pathway was enriched in the Notch signaling pathway; Survival analysis found that the high expression of SP8 transcription factor (SP8) gene leads to a decrease in overall survival in elderly patients with gastric cancer. Conclusion: The signal pathway and SP8 gene enriched in elderly gastric cancer analyzed by bioinformatics methods in this study may have a role in the development of gastric cancer in the elderly. |
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