| 王若男,赵德喜,孙晓舟,崔洪玉,吴九如.红细胞诱导BV-2小胶质细胞制作脑出血吞噬模型的研究[J].,2020,(6):1038-1042 |
| 红细胞诱导BV-2小胶质细胞制作脑出血吞噬模型的研究 |
| RBC Induced Microglia of BV-2 to Produce Intracerebral Hemorrhage Phagocytosis Model |
| 投稿时间：2019-10-27 修订日期：2019-11-22 |
| DOI：10.13241/j.cnki.pmb.2020.06.008 |
| 中文关键词: 红细胞 小胶质细胞 脑出血 吞噬 |
| 英文关键词: RBC BV-2 Intracerebral Hemorrhage Swallowing |
| 基金项目:国家自然科学基金面上项目（81774224）；吉林省教育厅"十三五"科学技术项目（JJKH20181247KJ） |
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| 中文摘要: |
| 摘要 目的：观察不同储存时间、不同比例的红细胞（RBC）与小鼠BV-2小胶质细胞共培养不同时间对BV-2吞噬RBC的影响。方法：将用阿氏液保存1天、7天、14天、21天、30天的小鼠RBC按照5：1、10：1、20：1、40：1的比例加入BV-2小胶质细胞中，共培养1 h、3 h、6 h、9 h， 应用流式细胞术观察 BV-2对于RBC的吞噬情况。结果：加入不同保存时间的RBC与BV-2小胶质细胞共培养均可见到吞噬情况，其中保存28天的效果最明显；加入不同比例的RBC与BV-2小胶质细胞共培养，均出现吞噬情况，其中40：1最明显；RBC与BV-2小胶质细胞共培养各时间点均可观察到吞噬情况，其中6小时最明显。结论：应用RBC诱导小鼠BV-2小胶质细胞制作大鼠脑出血体外模型，选择保存28天RBC与BV-2小胶质细胞的比例为40:1共培养6小时，此时BV-2小胶质细胞吞噬清除RBC的效果最明显。 |
| 英文摘要: |
| ABSTRACT Objective: Observe the influences that BV-2, co-cultured with mice' glial cell for different time, exerts to the swallowing RBC with different storage time and different proportion. Methods:Store mice' RBC with flow cytometry for 1 day, 7 days, 14 days, 21 days and 30 days respectively. Add the stored RBC into BV-2 glial cell with the proportion of 5:1, 10:1, 20:1 and 40:1. Co-culture them for 1 h, 3 h, 6 h, 9 h, and observe the swallowing condition between BV-2 and RBC. Results: The swallowing phenomenon can be observed after adding RBC with different stored time into BV-2 glial cell. The phenomenon is the most obvious after storing for 28 days; After co-culturing BV-2 and RBC with different proportion, the swallowing phenomenon is also observed. In which the phenomenon is the most obvious with the proportion of 40:1. The swallowing phenomenon can be observed in each co-culture time, in which the phenomenon is the most obvious after 6 h. Conclusion: Apply RBC to induce BV2 glial cell making in vitro intracerebral hemorrhage model. Choose RBC which stored for 28 days and co-culture it with BV-2 for 6h with proportion of 40:1. Thus, the swallowing phenomenon between BV2 glial cell and RBC is the most obvious. |
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