| 雍 陟,沈曼茹,高振军,颜美珠,倪倩雯,黄继英.小檗碱对肝硬化大鼠肠粘膜及肝脏保护作用的研究[J].,2020,(5):813-817 |
| 小檗碱对肝硬化大鼠肠粘膜及肝脏保护作用的研究 |
| Protective Effects of Berberine on the Intestinal Mucosal Barrier and Liver Function in Rats with Liver Cirrhosis |
| 投稿时间：2019-09-28 修订日期：2019-10-23 |
| DOI：10.13241/j.cnki.pmb.2020.05.003 |
| 中文关键词: 小檗碱 肝硬化 肠粘膜 肝脏保护 |
| 英文关键词: Berberine Liver cirrhosis Intestinal mucosa Liver protection |
| 基金项目:上海市青浦区科学技术委员会基金项目（QKY2018-06）；上海市卫健委基金项目（201940371） |
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| 中文摘要: |
| 摘要 目的：观察小檗碱对肝硬化大鼠肝脏和肠粘膜屏障的保护作用。方法：24只Wistar 大鼠随机分为小檗碱干预组、肝硬化造模组、正常对照组。小檗碱干预组和肝硬化造模组予以四氯化碳联合酒精进行肝硬化造模。小檗碱干预组造模同时予以小檗碱灌胃，观察大鼠的一般情况、进食量、体重，第8周末处死全部大鼠，检测血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、内毒素（ET）水平，并取肝组织和回肠进行病理检查。结果：肝硬化造模组大鼠和小檗碱干预组大鼠进食量及体重有下降，肝硬化造模组下降大于小檗碱干预组，差别有统计学意义P <0.01。 小檗碱干预组ALT，AST，ET水平均低于肝硬化造模组，差别有统计学意义P <0.01。肝脏病理观察：小檗碱干预组肝小叶结构破坏,假小叶形成，汇管区及小叶内炎症细胞浸润方面明显轻于肝硬化造模组。回盲部病理观察：小檗碱干预组的小肠绒毛缩短、变形、数量减少，上皮细胞排列紊乱方面轻于肝硬化造模组。结论：小檗碱对四氯化碳联合酒精所致的肝硬化大鼠有减轻肝损伤和保护肠粘膜的作用。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the protective effect of Berberine on intestinal mucosal barrier and liver function in rats with liver cirrhosis. Methods: A total of 24 male Wistar rats were randomly (random number) divided into 3 groups: model group (n=8), berberine group (n=8) and control group (n=8). The rats in the control group were injected intraperitoneally with saline daily. The rats in the model group were injected intraperitoneally with carbon tetrachloride to establish the model of liver cirrhosis. The rates in the berberine group were given berberine respectively based on the model group. The general condition of rats was observed. The body weight and diet intake of the rats in each group were recorded. All rats were sacrificed after 8 weeks and the plasma levels of endotoxin (ET), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. The pathological changes of liver and ileum tissue were observed by HE staining. Results: The rats in the model group and the berberine group had decreased food intake and body weight, and the decline in the model group was more significant than that in the berberine group (P<0.01). Compared with the model group, the serum levels of ALT, AST and LPS in the berberine group were significantly decreased (P<0.01). The pathological results showed that the structure of small intestinal villi, mucosal space and other structures in the model group were severely damaged, while the structures of villi and mucosal space were improved in the berberine group. Besides, model group presented disordered structure of liver, formation of pseudolobule and inflammatory cell infiltration in hepatic lobule while the structure of liver was improved in the berberine group. Conclusion: Berberine plays a protective role on the intestinal barrier and liver function in rats with liver cirrhosis induced by carbon tetrachloride and alcohol. |
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