文章摘要
金美君,李亮魁,李域琪,盛园园,徐 俐.Cidec基因敲除小鼠肠道菌群的特征[J].,2020,(3):401-407
Cidec基因敲除小鼠肠道菌群的特征
Composition of Gut Microbiota in Cidec-deficient Mice
投稿时间:2019-08-18  修订日期:2019-09-13
DOI:10.13241/j.cnki.pmb.2020.03.001
中文关键词: 肠道菌群  Cidec  高脂饮食
英文关键词: Gut microbiota  Cidec  High fat diet
基金项目:
作者单位E-mail
金美君 清华大学生命科学学院 北京 100084 18800116070@163.com 
李亮魁 清华大学生命科学学院 北京 100084  
李域琪 清华大学生命科学学院 北京 100084  
盛园园 清华大学生命科学学院 北京 100084  
徐 俐 清华大学生命科学学院 北京 100084  
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中文摘要:
      摘要 目的:探究Cidec敲除(CIDEC-KO)小鼠肠道菌群的结构。方法:随机分别挑选体重相近、2月龄5只野生型和5只Cidec敲除的雄性小鼠,收集两种基因型小鼠经高脂饲料16周喂养前后的新鲜粪便。提取粪便中的细菌基因组,对菌群基因组16S rRNA基因V4高变区进行测序,对数据进行PCoA分析、Alpha多样性分析及LEfSe分析。结果:属水平下的LEfSe分析显示,在普通饲料喂养条件下,Cidec缺失小鼠对比野生型小鼠粪便中Prevotellacea_UCG_001属丰度显著上升,Blautia属、Streptococcus属、Lachnospiraceae_UCG_006属丰度显著下降。与同月龄同高脂喂养的野生型小鼠比,Cidec敲除小鼠粪便中Ruminococcaceae_UCG_014属丰度显著下降。进一步比较同一种基因型下饮食对肠道菌群的改变,发现在喂养高脂饲料后,某些属的丰度仅在Cidec缺失小鼠中发生显著变化,但是在野生型小鼠中未发现有显著变化,这些属包括:Alistipes属、Bacteroides属、Paraprevotella属、Streptococcus属、Lachnospiraceae_UCG_006属丰度显著上升。而喂养高脂后,仅在野生型小鼠中发现Peptococcus属和Ruminococcus_torques_group属丰度的显著上升,以及Tyzzerella_3属、Ruminiclostidium_6属和A2属丰度的显著下降,在Cidec缺失小鼠粪便中并未发现这些属的丰度有显著变化。结论:高脂诱导下,对比野生型小鼠,某些同代谢综合征正相关的属只在Cidec缺失小鼠肠道菌群中丰度显著上升。
英文摘要:
      ABSTRACT Objective: To investigate the composition of gut microbiota in Cidec-deficient mice. Methods: Five wild-type (WT) and five Cidec-deficient (CIDEC-KO) male C57/B6J mice with same age (8-week-old) and similar weight were randomly selected. Fresh feces were collected before and after fed with high fat diets (HFD) for 16 weeks. Gut bacteria genome DNA was extracted for 16S rRNA V4 region sequencing. Principal Coordinates Analysis (PCoA), Alpha diversity and LEfSe analysis were performed. Results: LEfSe analysis revealed that in genus level, under normal diets (ND) condition, the abundance of the genus Blautia, Streptococcus, and Lachnospiraceae_UCG_006 in the feces of CIDEC-KO mice was lower, and the abundance of the genus prevotellacceae_UCG_001 was higher compared with WT mice. After fed with HFD, compared to WT mice, the abundance of the genus Ruminococcaceae_UCG_014 was lower in the feces of CIDEC-KO mice. To further compare the changes of intestinal flora after fed with HFD in CIDEC-KO mice, we found that the increase in the abundance of the genus Alistipes, Bacteroides, Paraprevotella, Streptococcus and Lachnospiraceae_UCG_006 could only be detected in the feces of CIDEC-KO mice but not in WT mice. Oppositely, the increase in the abundance of the genus Peptococcus and Ruminococcus_torques_group, and the decrease in the abundance of the genus Tyzzerella_3, Ruminiclostidium_6 and A2 could only be detected in WT mice, but not in CIDEC-KO mice after HFD. Conclusion: After fed with HFD for 16 weeks, the significant increase of several genera that were positively correlated with the metabolic syndrome could only be detected in the intestinal flora of CIDEC-KO mice not WT mice.
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