| 党学良,刘继洋,李文湖,杨 乐.亚麻木酚素对2型糖尿病小鼠学习记忆的影响及其机制初探[J].,2020,(2):259-263 |
| 亚麻木酚素对2型糖尿病小鼠学习记忆的影响及其机制初探 |
| Effects of Flax Lignans on Spatial Learning and Memory in Diabetic Encephalopathy Mice and Its Mechanism |
| 投稿时间：2019-06-30 修订日期：2019-08-01 |
| DOI：10.13241/j.cnki.pmb.2020.02.011 |
| 中文关键词: 亚麻木酚素 2型糖尿病 学习记忆 海马 突触可塑性 |
| 英文关键词: Flax lignans Type 2 diabetes mellitus Learning and memory Hippocampus Synaptic plasticity |
| 基金项目:陕西省重点研发一般项目（2018SF-305） |
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| 中文摘要: |
| 摘要 目的：研究亚麻木酚素（Flax ligands，FL）对2型糖尿病模型小鼠空间学习记忆的影响及其初步机制。方法：雄性C57小鼠随机分为对照组（Con）、糖尿病模型组（DM）及亚麻木酚素治疗组（DM+FL），DM与DM+FL组给予高脂饮食加小剂量链脲佐菌素（Streptozotocin，STZ）诱导2型糖尿病模型，之后DM+FL组灌胃给予FL 10 mg/kg，每日一次，连续14天，Con与DM组给予等量生理盐水。通过新物体识别试验、Morris水迷宫试验检测小鼠的学习记忆能力，利用Western blot技术测定小鼠海马脑源性神经营养因子（BDNF）及谷酰胺AMPA受体845位磷酸化（pGluA1-Ser845）蛋白表达水平。结果：与Con组比较，DM组小鼠新物体识别指数显著下降(P<0.01)，在Morris水迷宫中逃避潜伏期延长(P<0.05)，在目的象限内徘徊时间减少(P<0.01)；小鼠海马区BDNF和pGluA1-Ser845的蛋白表达水平均显著低于对照组（P<0.01）。与DM组相比，DM+FL组小鼠新物体识别指数显著提高(P<0.01)，在Morris水迷宫中逃避潜伏期明显缩短（P<0.05），目的象限徘徊时间显著增多（P<0.05）；小鼠海马区BDNF和pGluA1-Ser845的蛋白表达水平均显著升高（P<0.01）。结论：亚麻木酚素对2型糖尿病小鼠学习记忆有明确改善作用，增加海马BDNF和pGluA1-Ser845的表达可能是其潜在作用机制。 |
| 英文摘要: |
| ABSTRACT Objective: Study the effect and mechanism of Flax lignans on learning and memory in type 2 diabetic mice. Methods: Male C57 mice were randomly divided into 3 groups, control group (Con), type 2 diabetes mellitus group (DM) and Flax lignans treatment group (DM+FL). Except Con group, all mice were fed with high-fat diet and injected with streptozotocin intraperitoneally to induce type 2 diabetes mellitus, and the DM+FL mice were given 10 mg/kg FL by intragastric administration once daily for 14 days, mice in other two groups were given an equal volume of saline. New object recognition test and Morris water maze method were used to test the learning and memory function of mice, and protein levels of BDNF and pGluA1-Ser845 were detected by Western blot method. Results: Compared with the Con group, the recognition index was significantly decreased (P<0.01) in DM group during new object recognition test, the escape latency of the DM group mice was prolonged (P<0.05), and the wandering time in the target quadrant was reduced (P<0.01). Protein expression levels of BDNF and pGluA1-Ser845 in hippocampus of DM mice were significantly lower than those in Con group (P<0.01). Compared with the DM group, DM+FL group could increase the recognition index (P<0.01), reduce escape latency (P<0.05) and prolonged wandering time in the target quadrant (P<0.05). The protein expression levels of BDNF and pGluA1-Ser845 in hippocampus of DM+FL mice were significantly increased (P<0.01). Conclusion: Flax lignans could obviously improve the learning and memory in type 2 diabetic mice, and increasing protein levels of BDNF and pGluA1-Ser845 in hippocampus of DM mice may be the underlying mechanism. |
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