| 何 彩,奉 镭,石 蕾,徐骁骑,叶美玲.MiR-935通过其靶基因Notch1调控胃癌SGC7901细胞的发生发展[J].,2019,19(24):4628-4632 |
| MiR-935通过其靶基因Notch1调控胃癌SGC7901细胞的发生发展 |
| MiR-935 Regulates the Development of Gastric Cancer SGC7901 Cells through Its Target Gene Notch1 |
| 投稿时间：2019-07-23 修订日期：2019-08-18 |
| DOI：10.13241/j.cnki.pmb.2019.24.006 |
| 中文关键词: MiR-935 Notch1 胃癌 发生发展 |
| 英文关键词: MiR-935 Notch1 Castric cancer Occurrence and development |
| 基金项目:四川省卫生厅科研基金项目(140094) |
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| 中文摘要: |
| 摘要 目的：探究MiR-935调控胃癌SGC7901细胞的增殖和浸润与Notch1基因表达的关系。方法：分别检测40例正常人胃粘膜组织与40例胃印戒细胞癌的Notch1表达情况，并分析胃印戒细胞癌组织中Notch1表达与患者年龄、性别、组织进展程度、TNM分期、有无淋巴结转移的关系；采用MiR-935转染体外培养的SGC7901细胞系，检测Notch1的表达情况，其后采用MiR-935抑制剂处理，通过Transwell实验检测胃癌细胞的侵袭能力，细胞划痕实验检测胃癌细胞迁移能力。结果：正常人胃粘膜组织中Notch1表达呈阴性，而胃印戒细胞癌组织中Notch1表达呈阳性；Notch1的表达与胃印戒细胞癌的TNM分期、有无淋巴结转移存在着显著的相关性；转染MiR-935的SGC7901细胞Notch1表达明显上调，采用MiR-935抑制剂处理后，Notch1的表达显著下降。结论：MiR-935可能通过调控Notch1的表达调控胃癌的扩增和浸润。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the relationship between MiR-935 regulation of expansion and infiltration of gastric cancer SGC7901 cells and Notch1 gene expression. Methods: The expression of Notch1 in 40 normal gastric mucosa tissues and 40 signet ring cell carcinomas was detected, and the relationship between Notch1 expression and age, sex, tissue progression, TNM stage and lymph node metastasis was analyzed. The expression of Notch1 in SGC7901 cell lines was detected by MiR-935 in vitro. MiR-935 inhibitor was used to treat these transfected cell lines. Transwell test was used to detect the invasive ability of gastric cancer cells before and after transfection. Cell scratch test was used to detect the migration of gastric cancer cells before and after transfection. Change of migration ability and detection of Notch1 expression. Results: The expression of Notch1 was negative in normal gastric mucosa, but positive in signet ring cell carcinoma. After analysis, it was found that the expression of SIX was significantly correlated with TNM stage and lymph node metastasis of signet ring cell carcinoma. After transfection of SGC7901 cell line into MiR-935, the expression of Notch1 was significantly up-regulated, while after treatment with MiR-935 inhibitor, Notch1 was expressed. It showed a significant decline. Conclusion: Notch1 is overexpressed in human gastric signet ring cell carcinomas and promotes the proliferation and infiltration of cancer cells. MiR-935 regulates the proliferation and infiltration of gastric cancer by regulating Notch1 expression. |
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