| 王 景,张海丽,于 洋,郭伟光,黄敬文,安丽凤.全蝎软膏联用积雪苷软膏治疗增生性瘢痕的效果评价[J].,2019,19(22):4232-4237 |
| 全蝎软膏联用积雪苷软膏治疗增生性瘢痕的效果评价 |
| Evaluation of the Effect of Scorpion Ointment Combined with Asiaticoside Ointment in the Treatment of Hypertrophic Scar |
| 投稿时间：2019-09-23 修订日期：2019-10-20 |
| DOI：10.13241/j.cnki.pmb.2019.22.006 |
| 中文关键词: 全蝎软膏 积雪苷软膏 增生性瘢痕 胶原蛋白 TGF-β信号通路 |
| 英文关键词: Scorpion ointment Asiaticoside ointment Hypertrophic scar Collagen protein TGF-β signaling pathway |
| 基金项目:黑龙江省自然科学基金项目（H2017067; H201477）；黑龙江省博士后科研启动金项目（LBH-Q18126）；黑龙江省教育厅科学技术项目(12541765)；黑龙江中医药大学校基金（201812） |
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| 中文摘要: |
| 摘要 目的：观察全蝎软膏联用积雪苷软膏在早期治疗增生性瘢痕的治疗效果，并进一步探对组织中TGF-β信号通路的影响。方法：选取新西兰大白兔建立兔耳增生性瘢痕模型，随机平均分为对照组、模型组、全蝎软膏组、积雪苷软膏组和联合用药组；术后立即在治疗组患处涂抹全蝎软膏和/或积雪苷软膏，空白组和模型组涂抹PBS处理，连续用药35天并拍照记录各组瘢痕的治疗情况；于术后35 d收集各组兔耳组织样本进行病理检测、荧光定量PCR和western blot检测。结果：模型组于术后35天可见明显增生性瘢痕，各治疗组中联合用药组比单独药物治疗组的抑制效果更为显著，经治疗后无增生性瘢痕出现。H.E染色结果显示，与模型组相比，各治疗组经治疗后组织纤维化程度均减轻，胶原蛋白量均有不同程度降低，但联合用药可显著改善组织纤维化程度。荧光定量PCR和western blot检测结果表明，与模型组相比，治疗组组织内的MMP水平均呈上升趋势，Ⅰ型胶原蛋白（Col Ⅰ）、Ⅲ型胶原蛋白（Col Ⅲ）及Samd 4蛋白水平均不同程度下降，联合用药组差异最为显著，治疗效果优于单独用药组。结论：全蝎软膏联用积雪苷软膏可有效改善组织内细胞纤维化水平并减少胶原蛋白的沉积，可通过Smad 4蛋白的表达量调控TGF-β信号通路而抑制增生性瘢痕的形成。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the early effect of scorpion ointment combined with asiaticoside ointment on hypertrophic scar and the effect of TGF-β signaling pathway in tissues was further explored. Methods: The New Zealand white rabbits were randomly divided into control group, model group, scorpion ointment group, asiaticoside ointment group and combined drug group. Scorpion ointment and/or asiaticoside ointment were applied immediately after operation in the treatment group for 35 consecutive days, and the treatment of scars in each group was recorded by photography. Pathological examination, real-time PCR and Western blot were performed on rabbit ear tissue samples of each group 35 days after operation. Results: Typical hypertrophic scars were observed in model group 35 days after operation and the inhibitory effect of combined drug treatment group was more significant than that of single drug treatment group. The results of H. E staining showed that compared with the model group, the degree of tissue fibrosis and the amount of collagen in each treatment group were reduced to some extent after treatment, but the combined use of drugs could significantly improve the degree of tissue fibrosis. The results of real-time PCR and western blot showed that the MMP level in the treated group was higher than that in the model group, and the levels of type I collagen (Col I), type III collagen (Col III) and Samd 4 protein. The differences were all different, and the difference in the combination group was the most significant, and the treatment effect was better than that in the single drug group. Conclusion: The combination of scorpion ointment and asiaticoside ointment can effectively improve the level of cell fibrosis and reduce the deposition of collagen. The expression of Smad 4 protein can regulate the TGF-β signaling pathway and inhibit the formation of hypertrophic scar. |
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