文章摘要
白岭晓,宫 梅,孟江涛,王晓霞,丽 娜.EPO对肾脏缺血再灌注损伤大鼠肺内氧化应激状态的影响[J].,2019,19(22):4228-4231
EPO对肾脏缺血再灌注损伤大鼠肺内氧化应激状态的影响
Effects of EPO on Oxidative Stress in Lung of Rats with Renal Ischemia-reperfusion Injury
投稿时间:2019-05-05  修订日期:2019-05-28
DOI:10.13241/j.cnki.pmb.2019.22.005
中文关键词: 促红细胞生成素  大鼠  肾脏再灌注损伤  肺内氧化应激
英文关键词: Erythropoietin  Rat  Renal reperfusion injury  Intrapulmonary oxidative stress
基金项目:内蒙古自治区科技计划项目(20150352)
作者单位E-mail
白岭晓 内蒙古自治区人民医院重症医学科 内蒙古 呼和浩特010017 nmbailing_1970@163.com 
宫 梅 内蒙古自治区人民医院重症医学科 内蒙古 呼和浩特010017  
孟江涛 内蒙古自治区人民医院感染科 内蒙古 呼和浩特010017  
王晓霞 内蒙古自治区人民医院重症医学科 内蒙古 呼和浩特010017  
丽 娜 内蒙古自治区人民医院重症医学科 内蒙古 呼和浩特010017  
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中文摘要:
      摘要 目的:探讨促红细胞生成素(erythropoietin,EPO)对大鼠肾脏再灌注损伤模型肺内氧化应激状态的影响。方法:清洁级Sprague-Dawly(SD)大鼠36只适应性喂养1周后,随机分为3组,即假手术组(A组)、肾脏再灌注损伤组(B组)和EPO预处理组(C组),每组12只。A组大鼠只打开腹腔,游离双侧肾蒂但不夹闭;B组与C组都建立了大鼠肾脏再灌注损伤模型,且C组在夹闭肾蒂前2 h腹腔注射人重组EPO(5000 U/kg)。术后24 h,处死大鼠,检测肺内氧化应激水平。结果:A组大鼠精神状态良好,肾小管结构正常,未见明显上皮细胞肿胀、脱落,肺泡结构基本完整,肺泡间隔未增厚,有少量炎性细胞浸润;B组鼠毛耸立,无光泽,饮水量减少,肾小管结构破坏消失,肾小管扩张,可见大量蛋白管型,肺泡结构破坏,肺泡腔缩窄,肺泡间隔增厚,组织水肿,大量炎性细胞浸润;C组大鼠精神状态有所恢复,一般状况尚可,肾小管损伤较B组轻似,肾小管坏死区域有所减少,坏死偶见,肺泡壁轻度破坏,结构较为清晰,可见少量炎性细胞浸润。B组与C组大鼠的血尿素氮(blood urea nitrogen, BUN)与血肌酐(serum creatinine, Scr)水平、肺组织血红素氧合酶(heme oxygenase, HO)-1与丙二醛(malondialdehyde, MDA)水平都显著高于A组,C组以上指标均显著低于B组(P<0.05)。B组超氧化物歧化酶(superoxide dismutase, SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)的水平均显著高于A组(均P<0.05),而C组SOD和GSH-Px的水平均显著高于B组(均P<0.05),A组与C组间对比无显著差异。结论:EPO用于大鼠肾脏再灌注损伤模型能缓解肺内氧化应激状态,促进肾功能及肺组织恢复,发挥肾脏保护作用。
英文摘要:
      ABSTRACT Objective: To investigate the effects of erythropoietin (EPO) on oxidative stress in the lung of rats with renal reperfusion injury. Methods: 36 healthy Sprague-Dawly (SD) rats were randomly divided into 3 groups: sham operation group (group A), renal reperfusion injury group (group B) and EPO pretreatment group (group C), there were 12 rats in each group. Rats in group A were only opened the abdominal cavity, free bilateral renal pedicle but not clamped; group B and group C were established rat kidney reperfusion injury model, and group C were injected intraperitoneally with human recombinant EPO (5000 U/kg) at 2 hours before clamping the renal pedicle. All the rats were sacrificed 24 hours after surgery and were to detect the level of oxidative stress in the lung. Results: The rats in group A were in good mental state, normal tubular structure and there were no obvious swelling and shedding of epithelial cells, the alveolar structure was basically intact, the alveolar space was not thickened, and there was a small amount of inflammatory cell infiltration. The rats in group B were hairy, dull, water consumption decreased, renal tubular structure disappeared, renal tubules dilated, and there large number of protein casts were observed, and alveolar structure destruction, alveolar cavity narrowing, alveolar septum thickening, tissue edema, massive inflammatory cell infiltration were observed. The mental state of the rats in group C were recovered, the general condition were acceptable, the tubular damage was lighter than that of group B, the area of renal tubular necrosis were reduced, and necrosis were occasionally seen, the alveolar wall was slightly damaged, the structure was clear, and a small amount of inflammatory cell infiltration was observed. The levels of blood urea nitrogen (BUN) and serum creatinine (Scr), the levels of heme oxygenase(HO)-1 and malondialdehyde (MDA) in lung tissue in group B and group C were significantly higher than those in group A, and group C were significantly lower than group B (P<0.05). The levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in group B were significantly higher than those in group A (P<0.05), while the levels of SOD and GSH-Px in group C was significantly higher than those in group B (P<0.05). There was no significant difference between group A and group C. Conclusion: The application of EPO in the rat kidney reperfusion injury model can alleviate the oxidative stress in the lungs and promote the recovery of renal function and lung tissue, thus exerting renal protection.
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