| 章 萧,艾 芬,张莉红,刘 迪,张佩雯.微小RNA-30e调控EMT对胃癌侵袭和迁移影响的研究[J].,2019,19(22):4208-4212 |
| 微小RNA-30e调控EMT对胃癌侵袭和迁移影响的研究 |
| Effects of microRNA-30e Regulating Epithelial Mesenchymal Transition on Invasion and Migration in Gastric Cancer |
| 投稿时间：2019-05-08 修订日期：2019-05-30 |
| DOI：10.13241/j.cnki.pmb.2019.22.002 |
| 中文关键词: 胃癌 上皮细胞间充质转化 微小RNA-30e 侵袭 迁移 |
| 英文关键词: Gastric cancer EMT miR-30e Invasion Migration |
| 基金项目:湖北省卫生计生委科研项目（WJ2016MB007） |
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| 中文摘要: |
| 摘要 目的：探讨微小RNA-30e（miR-30e）对胃癌细胞迁移和侵袭能力的影响及可能的作用机制。方法：利用Transwell实验和细胞划痕实验检测胃癌细胞系BGC823侵袭和迁移的能力；以脂质体包裹合成miR-30e转染至BGC823细胞，并设空白载体作为对照组；Real-time PCR分别检测实验组和对照组细胞中miR-30e的表达。RT-PCR检测过表达miR-30e后对上皮细胞间充质转化（EMT）相关标记分子Snail、Vimentin、N-cadherin和E-cadherin表达的影响。结果：miR-30e转染至胃癌细胞后，抑制EMT通路主要因子Snail，Vimentin 和N-cadherin mRNA和蛋白质表达，而增加E-cadherin的mRNA和蛋白质表达；miR-30e通过TGF-β对BGC823细胞的侵袭和迁移能力有明显的抑制作用。结论：miR-30e可能是肿瘤细胞EMT过程的关键靶标靶点，阻断EMT过程，可以抑制胃癌细胞的侵袭和迁移能力。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of microRNA-30e (miR-30e) on the invasion and migration of gastric cancer and the potential mechanism. Methods: The transwell system and the assays of wound healing were used to assess the invasion and migration ability of gastric cell line BGC823. The miR-30e was transfected into BGC823 cell via lipofectamine 2000. Cells were also transfected with empty vectors to serve as controls. The expression of miR-30e was detected by real-time PCR, and western blot was used to assay the expression of Snail, Vimentin, N-cadherin, and E-cadherin which were the markers of epithelial-mesenchymal transition（EMT）. Results: After the transfection of the miR-30e, which had significantly suppressed the major factors in EMT signaling pathway, such as the expression of mRNA and proteins of Snail, N-cadherin, and vimentin, increased the mRNA and proteins expression of E-cadherin. And the mimic also had suppressed the invasion and migration of BGC823 cell line induced by TGF-β. Conclusion: MiR-30e may be a key target in the EMT process of cancer cells, blocking the EMT process could inhibit the invasion and migration of gastric cancer cells. |
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