| 王来藏,李晨光,谢 晨,邵正凯,李建华,李俞辰,王雪峰,张伟光.自噬在声动力疗法抑制C6胶质瘤细胞增殖中的作用[J].,2019,19(19):3616-3620 |
| 自噬在声动力疗法抑制C6胶质瘤细胞增殖中的作用 |
| The Role of Autophagy in the Inhibition of Proliferation of C6 Glioma Cells by Sonodynamic Therapy |
| 投稿时间：2019-07-01 修订日期：2019-07-23 |
| DOI：10.13241/j.cnki.pmb.2019.19.004 |
| 中文关键词: 自噬 声动力疗法 C6胶质瘤细胞 微管相关蛋白1轻链3 Beclin1 |
| 英文关键词: Autophagy Sonodynamic therapy C6 glioma cells Microtubule-associated protein 1 light chain 3 Beclin 1 |
| 基金项目:黑龙江省自然科学基金项目(H2016032)；国家自然科学基金青年项目(81702462) |
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| 中文摘要: |
| 摘要 目的：探讨自噬在血卟啉单甲醚(Hematoporphyrin monomethyl ether，HMME)介导的声动力疗法(Sonodynamic therapy，SDT)抑制C6胶质瘤细胞增殖中的作用。方法：选取对数期生长的C6胶质瘤细胞并随机分为四组：对照组（未予处理）、超声组（单独超声照射）、HMME组（单独加入HMME）、SDT组(超声照射+HMME)。透射电镜观察SDT处理的C6胶质瘤细胞中自噬体数量的改变。应用qRT-PCR和免疫印迹分析SDT处理对C6胶质瘤细胞中的LC3、Beclin1、Bcl-2 mRNA及蛋白表达水平的影响。MTT检测C6胶质瘤细胞的活力变化。结果：透射电子显微镜显示SDT组自噬体数量较对照组明显增多。SDT组C6胶质瘤细胞中微管相关蛋白1轻链3(Microtubule associated protein 1 light chain 3, LC3)、Beclin1 mRNA和蛋白水平高于对照组，B细胞淋巴瘤-2(B cell lymphoma-2, Bcl-2) mRNA和蛋白水平低于对照组。与对照组相比，SDT组C6胶质瘤细胞存活率从0 h至6 h逐渐下降，从12 h至72 h逐渐升高。3-甲基腺嘌呤(3-Methyladenine,3-MA)+SDT、氯喹(Chloroquine,CQ)+SDT处理后C6胶质瘤细胞存活率较SDT组明显降低。结论：SDT可能通过诱导自噬抑制C6胶质瘤细胞增殖。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the role of autophagy in Hematoporphyrin monomethyl ether (HMME)-mediated Sonodynamic therapy (SDT) in inhibiting the proliferation of C6 glioma cells. Methods: C6 glioma cells grown in log phase were randomly divided into four groups: control group (not treated), ultrasound group (individual ultrasound irradiation), HMME group (HMME alone), SDT group (ultrasound irradiation + HMME). The number of autophagic vacuoles in SDT treated C6 glioma cells was observed by transmission electron microscopy. The effects of SDT treatment on the expression of LC3, Beclin1, Bcl-2 in C6 glioma cells were analyzed by qRT-PCR and Western blot. MTT was used to detect the viability of C6 glioma cells. Results: Transmission electron microscopy analysis demonstrated that the number of autophagy in SDT group was significantly higher than that in the control group. The mRNA and protein levels of microtubule associated protein 1 light chain 3(LC3) and Beclin1in C6 glioma cells of SDT group were higher than those of the control group, and the levels of B cell lymphoma-2(Bcl-2) mRNA and protein were lower than those of the control group. Compared with the control group, the viability of C6 glioma cells in SDT group decreased from 0 h to 6 h, and increased from 12 h to 72 h. The viability of C6 glioma cells after 3-methyladenine (3-MA)+SDT and Chloroquine (CQ)+SDT treatment was significantly lower than that of SDT group. Conclusion: SDT may inhibit the proliferation of C6 glioma cells by inducing autophagy. |
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