| 黄 达,罗 华,王 炜,宋志勇,许海霞,蔡维君,罗明英.小鼠胸主动脉血管管壁结构和平滑肌细胞表型的年龄变化[J].,2019,19(17):3213-3217 |
| 小鼠胸主动脉血管管壁结构和平滑肌细胞表型的年龄变化 |
| Changes of Vascular Wall Structure and Smooth Muscle Cell Phenotype in the Thoracic Aorta of Mice during Development, Maturation and Ageing |
| 投稿时间：2019-04-23 修订日期：2019-05-17 |
| DOI：10.13241/j.cnki.pmb.2019.17.003 |
| 中文关键词: 胸主动脉 平滑肌细胞 老化 |
| 英文关键词: Thoracic aorta Smooth muscle cell Aging |
| 基金项目:国家自然科学基金项目(81500377) |
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| 中文摘要: |
| 摘要 目的：通过研究小鼠胸主动脉血管直径和管壁厚度以及平滑肌细胞表型标志物波形蛋白(Vimentin)和平滑肌细胞肌动蛋白(α-SM-actin)的表达，探讨胸主动脉在发育、成熟和老化过程中血管管壁结构和平滑肌细胞表型的变化。方法：将24只昆明小鼠按年龄分成4组：3天组、3个月组、6个月组和16个月组。采用HE染色和免疫荧光术分别观察胸主动脉血管管壁结构以及Vimentin和α-SM-actin的表达变化。结果：随着年龄的增长，胸主动脉血管直径和管壁厚度增加，以16个月小鼠胸主动脉的直径最大和管壁最厚，但细胞密度减少。3天小鼠胸主动脉的Vimentin表达较高，6个月表达下降，16个月重新上调；而α-SM-actin在3天小鼠的胸主动脉表达较低，6个月表达增加，16个月表达出现下调，其差异均有统计学意义(P<0.05)。结论：小鼠胸主动脉的直径和管壁的厚度随年龄的增长而增加，而细胞的密度则随着年龄增加而减少；平滑肌细胞的表型从幼龄时的合成表型转变为收缩表型，老龄时又转变为合成表型。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the changes of vascular wall structure and smooth muscle cell phenotype during the development, maturation and aging of the thoracic aorta, by studying the diameter of the thoracic aorta and the wall thickness of mice and the smooth muscle cell phenotype markers of vimentin and α-SM-actin. Methods: Twenty-four Kun-Ming mice were divided 4 groups according to their ages: 3 d, 3 m, 6 m and 16 m. The following experiments were performed HE staining for measurements of the diameter and thickness of the thoracic aorta. The expression of Vimentin and α-SM-actin was detected by immunofluorescence with special antibodies against Vimentin and α-SM-actin. Results: Thoracic aortic diameter and wall thickness were higher in 16 m mice and the cellular density decreased. In 3 d mice, vimentin expression in the thoracic aorta was higher, and decreased in 6 m old mice, then up-regulated in 16 m mice. In 3 d mice, α-SM actin expression in the thoracic aorta was lower, and increased in 6 m mice, then down-regulated in 16 m mice(P<0.05). Conclusion: The diameter of the thoracic aorta and the wall thickness of the Kunming mice increase with age, and the cellular density was reduced in the medial layer of aortas from the old mice. The phenotype of smooth muscle cells changes from a synthetic phenotype in young to a contractile phenotype, which in turn changes to a synthetic phenotype in age. |
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