| 白 颖,赵丹丹,朱如愿,柳辰玥,刘海霞,秦有文,高思华.降糖3号方对肥胖小鼠糖脂代谢及PI3K/AKT信号通路的影响[J].,2019,19(16):3039-3043 |
| 降糖3号方对肥胖小鼠糖脂代谢及PI3K/AKT信号通路的影响 |
| Effects of Jiangtangsanhao Formula on Glucolipid Metabolism and PI3K/AKT Signaling Pathway in Obese Mice |
| 投稿时间：2019-03-25 修订日期：2019-04-22 |
| DOI：10.13241/j.cnki.pmb.2019.16.007 |
| 中文关键词: 降糖3号方 肥胖 糖脂代谢 PI3K/AKT信号通路 |
| 英文关键词: Jiangtangsanhao Formula Obesity Glycolipid metabolism PI3K/AKT Signaling Pathway |
| 基金项目:朝阳区科委协同创新项目（CYXC1513）；国家重大新药创制子课题（2012ZX09103201-005）；北京市共建项目（0101216-14＆0101216-2013） |
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| 中文摘要: |
| 摘要 目的：观察降糖3号方对肥胖模型小鼠的体重、血糖、血清胰岛素含量、糖耐量、血脂等指标的影响，并探讨其对骨骼肌PI3K/AKT信号通路的影响。方法：8周龄C57BL/6J小鼠采用高脂喂养12周的方式诱导肥胖模型，将成模小鼠随机分为模型对照组，二甲双胍组，降糖3号方组，同时以正常饲料喂养的小鼠作为正常对照，进行为期8周的药物干预。每2周测量小鼠的体重、空腹血糖；第7周末进行口服葡萄糖耐量实验。实验结束后进行取材，检测糖化血红蛋白（HbA1c）、胰岛素（Insulin）、总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）水平，免疫印迹法检测骨骼肌组织PI3K、AKT、GLUT4等蛋白的表达。结果：降糖3号方能明显减轻肥胖小鼠的体重（P <0.05）；能明显降低肥胖小鼠空腹血糖，使之恢复正常水平（P<0.05）；降糖3号方组小鼠糖化血红蛋白（HbA1c）、胰岛素（INS）、总胆固醇（TC）、低密度脂蛋白（LDL-C）等指标显著低于模型组（P <0.05），且能有效改善实验动物的糖耐量（P <0.05）。蛋白印迹法检测结果表明，与模型组相比，降糖3号方可上调PI3K、AKT、GLUT4等蛋白表达水平（P <0.05）。结论：降糖3号方能够有效减轻肥胖小鼠体重，改善糖脂代谢，降低胰岛素水平，其作用可能是通过激活PI3K/AKT信号通路来实现。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the effects of Jiangtangsanhao (JTSH) formula on body weight, blood glucose, serum insulin content, glucose tolerance and blood lipid in diet induced obese mice, and to explore its effects on PI3K/AKT signaling pathway in the skeletal muscle. Methods: Eight-week-old C57BL/6J mice were fed with high-fat feed for 12 consecutive weeks to induce obesity model. Then the mice were randomly divided into model control group, metformin group and JTSH formula group. The mice fed with normal diet were used as normal control group. All the mice received 8 weeks of intervention by gavage, and the normal and model group received the same amount of sterile water. Body weight and fasting blood glucose were measured every 2 weeks; oral glucose tolerance test was performed 7 weeks later. Samples were taken after the drug intervention and the levels of HbA1c, Insulin, TC, TG, LDL-C and HDL-C were detected. The expressions of PI3K, AKT and GLUT4 in skeletal muscle were detected by Western Blot. Results: JTSH formula could significantly reduce the body weight of obese mice (P < 0.05); it could also effectively reduce the fasting blood glucose of obese mice to the normal level (P < 0.05); As to indicators like HbA1c, INS, TC, LDL-C, the mice in JTSH formula group were much lower than the model group (P < 0.05). According to the results of oral glucose tolerance test (OGTT), the glucose tolerance of those experimental animals were improved after the intervention of JTSH formula (P < 0.05). Western blot analysis showed that JTSH formula could up-regulate the expression of PI3K, AKT, GLUT4 compared with model group (P < 0.05). Conclusion: JTSH formula can effectively reduce the weight of obese mice, improve the metabolism of glucose and lipid, and reduce the level of insulin. Its effect may be achieved by activating PI3K/AKT signaling pathway. |
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