| 段 凯,李小刚,黄国庆,易 峰,吴 磊.京尼平对脓毒血症小鼠T细胞的免疫调节作用及其潜在机制[J].,2019,19(15):2818-2821 |
| 京尼平对脓毒血症小鼠T细胞的免疫调节作用及其潜在机制 |
| Immunomodulatory Effect of Genipin on T Cell in Sepsis Mice and Its Potential Mechanism |
| 投稿时间：2019-02-11 修订日期：2019-02-28 |
| DOI：10.13241/j.cnki.pmb.2019.15.004 |
| 中文关键词: 京尼平 脓毒血症 盲肠结扎穿孔手术 T细胞 凋亡蛋白 细胞因子 |
| 英文关键词: Genipin Sepsis Cecal ligation and puncture T cell Apoptotic proteins Cytokines |
| 基金项目:湖南省卫计委科研项目（B20160725） |
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| 中文摘要: |
| 摘要 目的：探究京尼平对脓毒血症小鼠T细胞的免疫调节作用及其潜在机制。方法：小鼠盲肠结扎穿孔手术（CLP）后，分别在0h和24 h尾静脉注射1 mg/kg、2.5 mg/kg、5 mg/kg京尼平和磷酸盐缓冲液（PBS），连续7d观察和记录小鼠的死亡数。另取小鼠分为Sham组（n=8）、Genipin组（n=8）、CLP组（n=8）、Genipin+CLP组（n=14），Sham组注射PBS并实施假手术，Genipin组注射2.5 mg/kg京尼平并实施假手术，CLP组注射PBS并实施手术，Genipin+CLP组注射2.5 mg/kg京尼平并实施手术。CLP手术26 h后收集脾脏，检测脾脏淋巴细胞总数、CD4+、CD8+、细胞因子和凋亡蛋白FADD、caspase-3、caspase-8表达水平。结果：给药2.5 mg/kg的京尼平小鼠存活率高于其他小鼠。CLP组小鼠脾脏淋巴细胞总数、CD4+、CD8+、白介素（IL）-2、干扰素（IFN）-γ水平低于Sham组，CLP+Genipin组小鼠上述指标高于CLP组（P<0.05）；CLP组小鼠凋亡蛋白FADD、caspase-3、caspase-8表达、IL-4、IL-10水平高于Sham组，CLP+Genipin组小鼠上述指标低于CLP组（P<0.05）。结论：京尼平能够通过抑制T细胞凋亡改善脓毒血症后期的免疫抑制，提高脓毒性小鼠的存活率。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the immunomodulatory effect of genipin on T cell in sepsis mice and its potential mechanism. Methods: After cecal ligation and puncture (CLP) in mice, 1 mg/kg, 2.5 mg/kg, 5 mg/kg genipin and phosphate buffer (PBS) were injected into tail vein at 0 h and 24 h, respectively. Mortality of mice was observed and recorded for 7 consecutive days. The mice were divided into Sham group (n=8), Genipin group (n=8), CLP group (n=8), CLP and Genipin group (n=14). Sham group was injected with PBS and Sham operation was performed. Genipin group was injected with 2.5 mg/kg genipin and sham operation was performed. CLP group was injected with PBS and operated. CLP and Genipin group was injected with 2.5 mg/kg Genipin and operated. The spleens were collected 26 hours after CLP. The total splenic lymphocyte count, CD4+, CD8+, cytokines and apoptotic proteins FADD, caspase-3 and caspase-8 were detected. Results: The survival rate of genipin mice treated with 2.5 mg/kg was higher than that of other mice. The total splenic lymphocyte count, CD4+, CD8+, interleukin (IL)-2, interferon (IFN) -γ levels in CLP group were lower than those in Sham group and Genipin group, the above indexes in CLP group were higher than those in CLP group(P<0.05). The expression of apoptotic proteins FADD, caspase-3, caspase-8, IL-4 and IL-10 levels in CLP group were higher than those in Sham group, Genipin group, the above indexes in CLP group were lower than those in CLP group (P<0.05). Conclusion: Genipin can improve the immune suppression in late sepsis by inhibiting T cell apoptosis, and it can improve the survival rate of septic mice. |
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