| 王 晶,何学智,鲁希夷,张二宝,苗登顺.长非编码RNA SNHG18对人胃癌细胞BGC823增殖和凋亡的影响[J].,2019,19(15):2801-2806 |
| 长非编码RNA SNHG18对人胃癌细胞BGC823增殖和凋亡的影响 |
| Effect of Long non-coding RNA SNHG18 on the Proliferation and Apoptosis of Gastric Cancer BGC823 Cell Line |
| 投稿时间：2019-04-01 修订日期：2019-04-22 |
| DOI：10.13241/j.cnki.pmb.2019.15.001 |
| 中文关键词: 胃癌 lncRNA SNHG18 细胞增殖 细胞凋亡 |
| 英文关键词: Gastric cancer LncRNA SNHG18 Cell proliferation Cell apoptosis |
| 基金项目:国家自然科学基金重点项目(81730066)；中国江苏省研究生研究创新计划(KYCX17_1301) |
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| 中文摘要: |
| 摘要 目的：探究长非编码RNA SNHG18对胃癌细胞增殖和凋亡的影响。方法：采用实时定量PCR(qRT-PCR)技术检测人胃癌组织及癌旁组织和胃癌细胞系中lncRNA SNHG18的表达；采用MTT和克隆形成试验观察转染SNHG18过表达质粒后胃癌细胞BGC823增殖活力的变化；通过流式细胞术检测lncRNA SNHG18对胃癌细胞BGC823凋亡的影响。结果：相较于癌旁组织和胃正常粘膜上皮细胞系GSE-1，胃癌组织及胃癌细胞系中SNHG18的表达水平显著降低(P<0.05)；胃癌细胞过表达SNHG18增殖活力以及克隆形成的能力均显著降低(P<0.05)，而细胞凋亡率明显升高(P<0.05)。结论：胃癌组织中长非编码RNA SNHG18呈低表达，可促进胃癌细胞增殖并抑制其凋亡，可能在胃癌发生发展过程中发挥重要作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of long non-coding RNA SNHG18 on the proliferation and apoptosis of gastric cancer cells. Methods: Real-time quantitative PCR(qRT-PCR) was used to detect the expression of lncRNA SNHG18 in human gastric cancer tissues and adjacent normal tissues and gastric cancer cell lines. MTT and colony formation assays were used to observe the changes of proliferation activity of gastric cancer BGC823 cells after transfection of pcDNA-SNHG18. The effect of lncRNA SNHG18 on the apoptosis of gastric cancer cells was verified by flow cytometry. Results: Compared with paracancerous tissues and normal gastric mucosal epithelial cell line GSE-1, the expression of SNHG18 was down-regulated in gastric cancer tissues and gastric cancer cell lines (P<0.05); and over expression SNHG18 in gastric cancer cells could inhibit the proliferation of gastric cancer cell line BGC823 and pro- mote apoptosis of BGC823 cells. Conclusion: The expression of long non-coding RNA SNHG18 is down-regulated in the gastric cancer tissues, which may play an important role in the regulation of proliferation and apoptosis of gastric cancer BGC823 cells. |
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