文章摘要
王大秀,王 楠,李旭辉,闫小敏,周 婷,孟宪红,胡凤丽,韩继武.成纤维细胞生长因子21对非酒精性脂肪性肝炎的作用及机制的研究[J].,2019,19(13):2434-2437
成纤维细胞生长因子21对非酒精性脂肪性肝炎的作用及机制的研究
Effect of Fibroblast Growth Factor 21 on Non-alcoholic Steatohepatitis and Its Mechanism
投稿时间:2019-04-26  修订日期:2019-05-20
DOI:10.13241/j.cnki.pmb.2019.13.007
中文关键词: 成纤维细胞生长因子21  非酒精性脂肪性肝炎  核因子E2相关因子-2  核苷酸结合寡聚化结构域样受体3
英文关键词: Fibroblast growth factor 21  Non-alcoholic steatohepatitis  Nrf-2  NLRP3
基金项目:哈尔滨市科技局优秀学科带头人基金项目(2014RFXGJ043);黑龙江省教育厅科技研究项目资助面上项目(12521357)
作者单位E-mail
王大秀 哈尔滨医科大学附属第四医院消化内科 黑龙江 哈尔滨 150001 2691827406@qq.com 
王 楠 东北农业大学生命科学学院 黑龙江 哈尔滨 150030  
李旭辉 黑龙江省红十字医院(黑龙江省森工总医院) 黑龙江 哈尔滨 150040  
闫小敏 哈尔滨医科大学附属第四医院消化内科 黑龙江 哈尔滨 150001  
周 婷 哈尔滨医科大学附属第四医院消化内科 黑龙江 哈尔滨 150001  
孟宪红 哈尔滨医科大学附属第四医院消化内科 黑龙江 哈尔滨 150001  
胡凤丽 哈尔滨医科大学附属第四医院消化内科 黑龙江 哈尔滨 150001  
韩继武 哈尔滨医科大学附属第四医院消化内科 黑龙江 哈尔滨 150001  
摘要点击次数: 21
全文下载次数: 26
中文摘要:
      摘要 目的:探索成纤维细胞生长因子21(FGF-21)对非酒精性脂肪性肝炎(NASH)的作用及其机制。方法:用浓度为500 μmol/L的油酸和棕榈酸混合物(摩尔比=2:1)诱导HepG2细胞建立NASH细胞模型,实验分为5组:正常对照组(Control)、模型组(Model)、低剂量FGF-21组(LFGF-21,0.5 μmol/L)、中剂量FGF-21组(MFGF-21,1.0 μmol/L)和高剂量FGF-21组(HFGF-21,2.0 μmol/L),油红O染色法观察细胞内脂滴,全自动生化分析仪检测细胞内ALT、AST、TC、TG的水平,Real-time PCR和Western blot分别检测细胞内核因子E2相关因子-2(Nrf-2)、核苷酸结合寡聚化结构域样受体3(NLRP3)的mRNA和蛋白水平,ELISA检测IL-1β、TNF-α水平。结果:NASH细胞造模成功,油红O染色结果显示对照组细胞无明显脂滴蓄积,模型组细胞内可见大量橘红色脂滴,并出现融合现象。不同浓度FGF-21治疗组的细胞内红色脂滴明显减少,并呈剂量依赖性。模型组ALT、AST、TC、TG、IL-1β、TNF-α和NLRP3的水平均高于对照组(P<0.05),FGF-21治疗组其水平低于模型组(P<0.05)。模型组Nrf2的水平低于对照组(P<0.05),FGF-21治疗组Nrf2的水平高于模型组(P<0.05)。结论:FGF-21通过促进Nrf-2、抑制NLRP3减少非酒精性脂肪性肝炎细胞的脂质沉积,减轻炎症反应,对NASH有保护作用。
英文摘要:
      ABSTRACT Objective: To investigate the effect of fibroblast growth factor 21 (FGF-21) on non-alcoholic steatohepatitis(NASH) and the mechanism of its action. Methods: Culturing HepG2 cells with DMEM medium supplemented with 10% fetal bovine serum. HepG2 cells were randomly divided into five groups: control group, model group (500 μmol/L FFA), low dose FGF-21(LFGF-21, 0.5 μmol/L), middle dose FGF-21(MFGF-21, 1.0 μmol/L) and high dose FGF-21(HFGF-21, 2.0 μmol/L). Observing intracellular lipid droplets by oil red O staining, the levels of AST, ALT, TC and TG were measured by automatic chemistry analyzer. The mRNA contents and protein levels of Nrf-2 and NLRP3 were determined by Real-time PCR and Western blot. The protein levels of IL-1β and TNF-α were measured by ELISA kit. Results: The NASH cell model was established. The oil red O staining showed that no lipid droplets were found in the control group, and there were mass lipid droplets in the model group, and some were fused, and those in the treatment groups decreased significantly compared with the model group on dose-dependent. The levels of AST, ALT, TC, TG, IL-1β, TNF-α and NLRP3 in the model group were significantly higher than those in the control group (P<0.05), and those in the treatment group decreased significantly compared with the model group (P<0.05). The levels of Nrf-2 in the model group were significantly lower than those in the control group (P<0.05), and that in the treatment group increased significantly compared with the model group (P<0.05). Conclusion: FGF-21 can reduce lipid deposition and inhibit inflammation through activating Nrf-2 and inhibiting NLRP3 to attenuate steatohepatitis.
查看全文   查看/发表评论  下载PDF阅读器
关闭