| 卢志承,陈世梁,符志龙,吴文川,陈春吉.混合甲醛固定液固定大肠癌淋巴结标本的最佳免疫组化效果研究[J].,2019,19(12):2379-2382 |
| 混合甲醛固定液固定大肠癌淋巴结标本的最佳免疫组化效果研究 |
| Best Immunohistochemical Effect of Mixed Formaldehyde Fixative for Colorectal Cancer Lymph Node Specimens |
| 投稿时间：2018-10-31 修订日期：2018-11-24 |
| DOI：10.13241/j.cnki.pmb.2019.12.039 |
| 中文关键词: 混合甲醛固定液 不同pH值 不同时间 免疫组化 大肠癌淋巴结标本 细胞角蛋白20 |
| 英文关键词: Mixed formaldehyde fixative Different pH values Different time Immunohistochemical Colorectal cancer lymph node specimens Cytokeratin 20 |
| 基金项目:海南省医学科研基金资助项目（20140163） |
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| 中文摘要: |
| 摘要 目的：探讨混合甲醛固定液固定大肠癌淋巴结标本的最佳免疫组化效果。方法：采用不同pH值（6.0、7.0、8.0）的混合甲醛固定液对39枚大肠癌淋巴结标本进行不同时间（<6 h、6 h-12 h、1 d-7 d）的固定处理。以细胞角蛋白20（CK20）为目标抗原，运用OIympusdp 70图像采集分析仪抽选出混合甲醛固定液最佳免疫组化染色的pH值及固定时间。结果：结论：对于大肠癌淋巴结标本，以CK20为目标抗原，选择pH值为7.0的混合甲醛固定液固定6 h-12 h能够得到质量较佳的免疫组化染色效果。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the best immunohistochemical effect of mixed formaldehyde fixative for colorectal cancer lymph node specimens. Methods: A total of 39 cases of colorectal cancer lymph node specimens were fixed treatment at different time (<6 h, 6 h-12 h,1 d-7 d) by different pH values (6.0,7.0,8.0) of mixed formaldehyde fixative. The cytokeratin 20 (CK20) was used as the target antigen. OIympusdp 70 image acquisition analyzer was used to extract the best pH value and fixation time of mixed formaldehyde fixative. Results: After mixed formaldehyde fixative treated by pH values 7.0, the positive rate was 92.31%, which was higher than 76.92% and 74.36% of mixed formaldehyde fixative treated by pH values 6.0 and 8.0 respectively. And there were statistical differences between the positive rate after treated by pH values 7.0 and 8.0(P<0.05). The positive rate of mixed formaldehyde fixative at 6 h-12 h was 94.87%, higher than 30.77% and 76.92% at the fixed time less than 6 h and 1 d-7 d. Conclusion: For colorectal cancer lymph node specimens, CK20 is used as the target antigen, a better quality of immunohistochemical stain effect could be obtained by fixing for 6 h-12 h with mixed formaldehyde fixative of pH values 7.0. |
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