| 时浩清,訾晓渊,张春雷,杨 琦,孙颖浩.前列腺癌细胞系中环状RNA circRNA-1565的表达及鉴定[J].,2019,19(12):2243-2247 |
| 前列腺癌细胞系中环状RNA circRNA-1565的表达及鉴定 |
| Expression and Identification of Ring RNA CircRNA-1565 in Prostate Cancer Cell Line |
| 投稿时间：2019-04-10 修订日期：2019-04-28 |
| DOI：10.13241/j.cnki.pmb.2019.12.009 |
| 中文关键词: 前列腺癌 环状RNA circRNA 基因表达 |
| 英文关键词: Prostate cancer circRNA circular RNA Gene express |
| 基金项目:上海市浦江人才计划项目(16PJ1411100) |
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| 中文摘要: |
| 摘要 目的：探讨前列腺癌细胞系中的环状RNA circRNA-1565的表达及鉴定。方法：培养前列腺正常上皮细胞（RWPE-1）、4种前列腺癌细胞（22RV1、LNCap、PC-3、DU145），提取总RNA，采用RT-PCR检测不同细胞系中circRNA-1565的表达，并对其进行成环性验证及细胞内亚定位实验，进行差异比较。结果：circRNA-1565在正常前列腺细胞系 (RWPE-1) 中表达量极低，在转移性前列腺癌细胞系中高表达，在非转移性前列腺癌细胞系中低表达。且circRNA-1565是一条主要定位于细胞质内的具有有效环状结构的RNA分子。结论：circRNA-1565在不同前列腺癌细胞系中存在差异表达，可能会通过miRNA sponge途径发挥生物学作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression and identification of annular RNA circRNA-1565 in the cell line of prostate cancer. Methods: Normal prostate epithelial cells (RWPE-1), 4 kinds of prostate cancer cells (22RV1, LNCap, PC-3, DU145) were cultured, total RNA was extracted, and RT-PCR was used to detect the expression of circRNA-1565 in different cell lines. The cyclic verification and intracellular localization experiments were carried out to compare the differences. Results: The expression of circRNA-1565 in normal prostate cell line (RWPE-1) was very low, expressed highly in metastatic prostate cancer cell line, and low in non-metastatic prostate cancer cell line. And circRNA-1565 is a RNA molecule with an effective annular structure, which is mainly located in the cytoplasm. Conclusion: The expression of circRNA-1565 in different prostate cancer cell lines may play a biological role through the sponge pathway of miRNA. |
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