| 刘永飞,张 莉,白卫平,李 娟,张贺铭,高昌俊.电针对大鼠心肺复苏后脑损伤的保护作用及对海马炎性因子表达的影响[J].,2019,19(12):2228-2232 |
| 电针对大鼠心肺复苏后脑损伤的保护作用及对海马炎性因子表达的影响 |
| Protective Effect of Electroacupuncture on Brain Injury after Cardiopulmonary Resuscitation in Rats and Its Effect on Expression of Inflammatory Factors in Hippocampus |
| 投稿时间：2019-01-07 修订日期：2019-02-01 |
| DOI：10.13241/j.cnki.pmb.2019.12.006 |
| 中文关键词: 电针 心肺复苏 心脏骤停 水沟穴 内关穴 |
| 英文关键词: Electroacupuncture Cardiopulmonary resuscitation Cardiac arrest Shuigou Neiguan |
| 基金项目:国家自然科学基金项目（81571183） |
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| 中文摘要: |
| 摘要 目的：探讨电针对大鼠心肺复苏后脑损伤及海马炎性因子表达的影响。方法：雄性SD大鼠随机分三组：假手术组（Sham）、对照组(Control)、电针组(EA)。大鼠窒息8 min后进行心肺复苏，EA组于复苏同时在水沟、内关穴插入毫针并予以电针刺激，对照组仅在相同穴位插入毫针。计算大鼠复苏成功率，记录自主循环恢复时间，于复苏后24 h及72 h对大鼠进行神经功能缺损评分（NDS），水迷宫检测各组大鼠学习记忆能力，尼氏染色观察海马区神经元形态及存活数量，Western blot 检测海马区炎性因子表达。结果：与Sham组相比，对照组与EA组大鼠复苏后24 h、72 h NDS降低，学习记忆能力明显减低，两组海马CA1区细胞排列紊乱、神经元存活数量减少，IL-10表达降低、IL-1与IL-6表达升高（P＜0.05）。而与对照组相比，EA组大鼠复苏成功率有所提高，但无统计学意义，自主循环恢复时间明显缩短（P＜0.05）；复苏后24 h、72 h NDS评分提高（P＜0.05）；水迷宫第六天逃避潜伏期缩短、空间探索能力显著增强（P＜0.05）；海马CA1区细胞排列紊乱减轻，神经元存活数目增多；海马区炎性因子IL-1、IL-6表达降低，抗炎因子IL-10表达增多（P＜0.05）。结论：电针能够减轻大鼠心肺复苏后脑损伤，其保护作用可能与抑制炎性因子、促进抗炎因子表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of electroacupuncture(EA) on brain injury and expression of inflammatory factors in hippocampus after cardiopulmonary resuscitation in rats. Methods: Male SD rats were randomly divided into three groups: sham group, control group and EA group. Rats were asphyxiated for 8min before cardiopulmonary resuscitation (CPR). The EA group was inserted filiform needles in the shuigou and neiguan points and received electrical stimulation, while the control group was only inserted filiform needles at the same points. The survival rate of rat resuscitation and the recovery time of spontaneous circulation were recorded. Neurological deficit score (NDS) was measured at 24 h and 72 h after resuscitation. The spatial learning and memory ability of each group was tested by Morris water maze. Neuronal morphology and survival in hippocampus were observed by Nissl staining. The expression of inflammatory factors in hippocampus was detected by Western blot. Results: Compared with the sham group, the NDS of rats at 24 h and 72 h after resuscitation and the spatial learning and memory ability were significantly reduced in other two groups. In this two groups, hippocampal CA1 cells were disordered, meanwhile, the number of viable neurons, the expression of IL-10 were decreased, but IL-1 and IL-6 were increased when compared with sham group(P＜0.05). Compared with the control group, the survival rate was improved without statistically significant, the recovery time of spontaneous circulation was shortened(P＜0.05), the NDS was increased at 24 h and 72 h after resuscitation(P＜0.05), the escape latency of 6th day of water maze was shortened and the space exploration ability was promoted in the EA group(P＜0.05). The cell line in the hippocampal CA1 and the number of viable neurons in the EA group were significantly better than that of control group. IL-1 and IL-6 in the hippocampus of EA group were alleviated, while the expression of anti-inflammatory factor IL-10 was enhanced compared with the control group (P＜0.05). Conclusion: Electroacupuncture can alleviate brain injury after cardiopulmonary resuscitation in rats, and its protective effect may be related with inhibiting inflammatory factors and promoting the expression of anti-inflammatory factors. |
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