| 蒋荣芳,李莉华,凌宙贵,温中薇,韦晓谋.黄芪提取液治疗呼吸道合胞病毒感染小鼠的作用机制研究[J].,2019,19(11):2081-2085 |
| 黄芪提取液治疗呼吸道合胞病毒感染小鼠的作用机制研究 |
| Study on Role Mechanism of Astragalus Membranaceus Extract in Treating Infected Mice with Respiratory Syncytial Virus |
| 投稿时间：2019-04-24 修订日期：2019-05-20 |
| DOI：10.13241/j.cnki.pmb.2019.11.016 |
| 中文关键词: 黄芪提取液 呼吸道合胞病毒 辅助性T细胞 作用机制 |
| 英文关键词: Astragalus membranaceus extract Respiratory syncytial virus Helper T cells Mechanism |
| 基金项目:广西壮族自治区自然科学基金项目（2011GXNSFA018362） |
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| 中文摘要: |
| 摘要 目的：分析黄芪提取液对呼吸道合胞病毒（RSV）感染小鼠的作用机制。方法：将30只SD小鼠随机分为对照组、模型组和黄芪组各10只。模型组和黄芪组均滴鼻滴度为10-3.5 TCID50/mL的RSV病毒液，制备小鼠RSV模型，对照组滴鼻等量生理盐水。黄芪组腹腔注射黄芪注射液18 g/kg，每日1次，连用9 d；对照组和模型组小鼠腹腔注射等体积的蒸馏水。治疗后，检测小鼠血清白细胞介素（IL）-2、IL-23、IL-17、IL-6、干扰素（IFN）-γ、超氧化物歧化酶（SOD）、丙二醛（MDA）和一氧化氮（NO）水平，Western blot检测Toll样受体（TLR）7蛋白表达水平，HE染色观察肺组织形态。结果：与对照组相比，模型组小鼠IL-23、IL-17、IL-6、NO、MDA和TLR7水平显著升高（P<0.05），IL-2、SOD和IFN-γ水平显著下降（P<0.05）；与模型组相比，黄芪组小鼠的IL-23、IL-17、IL-6、NO、MDA和TLR7水平显著下降（P<0.05），IL-2、SOD和IFN-γ水平显著升高（P<0.05）。结论：黄芪提取液可以有效调节辅助性T细胞释放细胞因子，保护呼吸道合胞病毒感染引起的免疫系统功能紊乱。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the mechanism of action of Astragalus membranaceus (Huangqi) extract on mice infected with respiratory syncytial virus (RSV). Methods: 30 SD mice were randomly divided into control group, model group and Huangqi group. Model group and Huangqi group were given RSV virus solution with a nasal titer of 10-3.5 TCID50/mL, and a mouse RSV model was prepared. Control group was given the same amount of normal saline. Huangqi group was intraperitoneally injected with 18 g/kg A. membranaceus injection once/d for 9 d. Control group and model group were injected intraperitoneally with an equal volume of distilled water. After treatment, the levels of serum interleukin (IL)-2, IL-23, IL-17, IL-6, interferon (IFN)-γ, superoxide dismutase (SOD), mal- ondialdehyde (MDA) and nitric oxide (NO) were measured in mice. And the expression level of Toll-like receptor (TLR) 7 protein was detected by Western blot, and the morphology of lung tissue was observed by HE staining. Results: Compared with control group, the levels of IL-23, IL-17, IL-6, NO, MDA and TLR7 in model group were significantly increased (P<0.05) while the levels of IL-2, SOD and IFN-γ were significantly decreased (P<0.05). Compared with model group, the levels of IL-23, IL-17, IL-6, NO, MDA and TLR7 in Huangqi group were significantly decreased (P<0.05) while the levels of IL-2, SOD and IFN-γ were significantly increased (P<0.05). Conclusion: A. membranaceus extract can effectively regulate the release of cytokines by helper T cells and protect the immune system dysfunction caused by respiratory syncytial virus infection. |
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