| 吴 兵,李世升,肖商荣,马书元,张亚飞.CD47-siRNA通过调控ROS诱导食管癌细胞SEG-1凋亡[J].,2019,19(11):2061-2065 |
| CD47-siRNA通过调控ROS诱导食管癌细胞SEG-1凋亡 |
| CD47-siRNA Induces the Apoptosis of Esophageal Cancer Cells SEG-1 by Regulating ROS |
| 投稿时间：2018-11-28 修订日期：2018-12-23 |
| DOI：10.13241/j.cnki.pmb.2019.11.012 |
| 中文关键词: CD47-siRNA转染 食管癌细胞SEG-1 凋亡 活性氧簇 |
| 英文关键词: CD47-siRNA Esophageal cancer cells SEG-1 Apoptosis Reative oxygen species |
| 基金项目:国家自然科学基金项目（81701501） |
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| 中文摘要: |
| 摘要 目的：探讨CD47-siRNA对食管癌细胞SEG-1凋亡的影响及其机制研究。方法：Western blot法检测食管癌细胞SEG-1中CD47蛋白、促凋亡蛋白Bax和抗凋亡蛋白Bcl-2的表达；CCK-8法检测食管癌细胞SEG-1细胞活力；DCFDA细胞ROS检测试剂盒检测食管癌细胞SEG-1 中ROS水平。结果：CD47-siRNA转染组食管癌细胞SEG-1中CD47蛋白明显低于空载对照组(P< 0.05)；CD47-siRNA转染组食管癌细胞SEG-1细胞活力显著低于空载对照组(P<0.05)；CD47-siRNA转染组食管癌细胞SEG-1中促凋亡蛋白Bax表达显著高于空载对照组（P＜0.05），抗凋亡蛋白Bcl-2表达低于空载对照组(P<0.05)；CD47-siRNA转染食管癌细胞SEG-1组ROS水平明显高于空载对照组(P< 0.05)；与CD47-siRNA转染组比较，CAT (ROS抑制剂，5 mM/L, 6 h) 预处理增加了细胞活力；与CD47-siRNA转染组比较，CAT (ROS抑制剂，5 mM/L, 6 h)预处理显著降低食管癌细胞SEG-1中Bax蛋白表达以及增加抗凋亡蛋白Bcl-2表达。结论：CD47-siRNA转染通过上调食管癌细胞SEG-1内ROS水平促进食管癌细胞SEG-1凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To detect the effects of CD47-siRNA on the apoptosis of esophageal cancer cells SEG-1 and its mechanism. Methods: Western blot was used to detect the CD47 protein, pro-apoptosis Bax and anti- apoptosis Bcl-2; Cell Counting Kit-8（CCK-8）was used to detect cell viability; DCFDA cellular ROS detection kit was used to detect the ROS level of esophageal cancer cells SEG-1. Results: CD47 protein in CD47-siRNA transfected group was significantly lower than Vector-NC group (P<0.05). The cell viability in CD47-siRNA transfected group was significantly lower than Vector-NC group (P<0.05). The expression of pro-apoptotic protein Bax in CD47-siRNA transfected group was significantly higher than Vector-NC group (P<0.05), and the expression of anti-apoptotic protein bcl-2 was lower than Vector-NC group (P<0.05). The ROS level in CD47-siRNA transfected group was significantly higher than Vector- NC group (P<0.05). Compared with CD47-siRNA transfected group, pretreatment with CAT (ROS inhibitor, 5 mm /L, 6 h) increased the cell viability. Compared with CD47-siRNA transfected group, pretreatment with CAT (ROS inhibitor, 5 mm/L, 6 h) significantly reduced the expression of Bax protein and increased the expression of Bcl-2 protein. Conclusion: CD47-siRNA can promote the apoptosis of esophageal cancer cells SEG-1 through increasing the level of ROS in esophageal cancer cells SEG-1. |
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