| 常方照,程 宣,李 倩,唐劲天,陆宇燕.辣椒叶多糖抗氧化作用研究[J].,2019,19(11):2019-2024 |
| 辣椒叶多糖抗氧化作用研究 |
| Study on Antioxidation of Capsicum annuum Leaves Polysaccharide |
| 投稿时间：2018-11-30 修订日期：2018-12-26 |
| DOI：10.13241/j.cnki.pmb.2019.11.004 |
| 中文关键词: 辣椒叶 多糖 氧自由基 抗氧化活性 |
| 英文关键词: Capsicum annuum leaves Polysaccharide ROS Antioxidation activity |
| 基金项目:国家863计划项目（20141860082）；辣椒叶主要成分分离鉴定以及生物活性研究（20132001124） |
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| 中文摘要: |
| 摘要 目的：从辣椒叶中分离纯化活性多糖并考察其抗氧化活性。方法：采用水提醇沉法提取多糖，Sevag法脱蛋白,得辣椒叶粗多糖；分别使用超纯水、0.06 mol/L NaCl溶液、0.18 mol/L NaCl溶液作为洗脱液，通过DEAE-52离子交换柱色谱纯化得到三种辣椒叶多糖LD-0、LD-0.06、LD-0.18，测定多糖含量。DPPH、ABTS法检测多糖体外抗氧化作用。以小鼠血清、肝组织中总超氧化物歧化酶活性、过氧化氢酶活性及丙二醛含量为指标，考察小鼠灌胃LD-0.06多糖对脂质过氧化模型的影响。结果：辣椒叶粗多糖、LD-0、LD-0.06、LD-0.18多糖含量分别为9.92%、43.14%、82.97%、37.63%，其中LD-0.06多糖含量最高。体外抗氧化实验结果显示，三种结果均具备较好的清除能力，其中LD-0.06对ABTS+、DPPH?的清除效果最好，IC50值分别为0.58 mg/mL和0.60 mg/mL，结果与对照组在0.05水平具有显著性差异，说明辣椒叶多糖提取物是一种有效的天然抗氧化剂。在脂质过氧化模型小鼠体内，与模型组比较，LD-0.06多糖能显著增强小鼠血清和肝组织中的T-SOD与CAT活性，降低MDA的含量，且剂量越高，体内抗氧化能力越强。结论：辣椒叶多糖提取物具有一定的抗氧化作用，为进一步开发利用辣椒资源提供了理论依据。 |
| 英文摘要: |
| ABSTRACT Objective: To extract, purify polysaccharids from Capsicum leaves and to determinate the antioxidant activity. Methods: Crude Capsicum leaves polysaccharide was gained by boiling in hot-water, precipitating using ethanol, removing proteins with Sevage method. Three kinds of Capsicum leaves polysaccharides LD-0, LD-0.06, LD-0.18 were purified through DEAE-52 cellulose colume, us- ing ultrapure water, 0.06 mol/L NaCl solution and 0.18 mol/L NaCl solution as eluent respectively. The content of polysaccharides was determined by the experiments of ABTS radical and DPPH radical scavenging. The effects of low, medium and high dose LD-0.06 polysaccharides on lipid peroxidation model in mice were studied, the indicators included total superoxide dismutase, catalase and mal- ondialdehyde. Results: The contents of polysaccharides, LD-0, LD-0.06 and LD-0.18 were 9.92%, 43.14%, 82.97% and 37.63% respec- tively. Among them, LD-0.06 has the highest polysaccharide content. Antioxidant activity tests revealed that all three methods have good scavenging ability, and LD-0.06 has the best effect on ABTS and DPPH. The IC50 values were 0.58 mg/mL and 0.60 mg/mL, respectively. Compared with the model group, LD-0.06 polysaccharide can significantly enhance the activity of T-SOD and CAT in serum and liver tissue of mice, reduce the content of MDA, and has a significant dose-effect relationship. Conclusion: Capsicum leaf polysaccharide ex- tract has certain antioxidant effect. This study indicated that polysaccharide extract of Casicum annuum leaves had high anti-oxidant ac- tivity, which provided a theoretical basis for the further research of the comprehensive development of Caspsicum annuum resources. |
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