| 欧 斐,巩月英,张 丽,马 兰,宋 燕.不同全血过滤方法用于去白细胞血液制备的临床对照研究[J].,2019,19(9):1774-1777 |
| 不同全血过滤方法用于去白细胞血液制备的临床对照研究 |
| Clinical Comparative Study of Different Whole Blood Filtration Methods for Blood Preparation of Leukocytes |
| 投稿时间：2018-09-05 修订日期：2018-09-28 |
| DOI：10.13241/j.cnki.pmb.2019.09.038 |
| 中文关键词: 全血过滤方法 去白细胞血液制备 临床对照研究 |
| 英文关键词: Whole blood filtration method Leukocyte-free blood preparation Clinical control study |
| 基金项目:青海省科学技术厅科研基金项目(9632015Y0147) |
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| 中文摘要: |
| 摘要 目的：观察不同全血过滤方法用于去白细胞血液制备的效果。方法：采用两种全血过滤方法进行对比研究，对照组采用常规法，将采集后全血混匀后直接与白细胞滤器连接直接过滤；实验组采用湿润滤盘法，血液采集完成混匀后静置，先用上层血清10~20 mL湿润滤盘，再混匀与白细胞滤器连接后进行过滤。比较两组制备方法所用的过滤时间、血液回收率、过滤前后血液指标情况及24小时内溶血的发生情况。结果：两组全血过滤方法过滤前后白细胞、红细胞、血红蛋白、血小板及血浆游离血红蛋白水平比较差异均无明显统计学意义(P>0.05)。而实验组过滤时间短于对照组，血液回收率高于对照组，且24小时内溶血比例明显低于对照组(P＜0.05)。结论：常规法与湿润滤盘法均能达到去白细胞血液标准，但湿润滤盘法较常规法能有效的降低过滤时间、增加血液回收率，减少去白细胞悬浮红细胞因溶血造成的血液不合格率，值得临床推广应用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of different whole blood filtration methods on the blood preparation of leukocytes. Methods: Two kinds of whole blood filtration methods were used for comparison. The control group adopted the routine method. After mixing the whole blood, the whole blood was mixed directly with the leucocyte filter. The test group adopted the wet filter plate method. The blood collection was finished after mixing, and the upper serum 10~20 mL was used to moisten the filter plate and then mixed with the white cell filter. The filtration time, blood recovery rate, blood indexes before and after filtration and the incidence of hemolysis with- in 24 hours were compared between the two groups. Results: There was no significant difference in the levels of white blood cells, red blood cells, hemoglobin, platelets and plasma free hemoglobin between the two groups before and after whole blood filtration (P>0.05). While the filtration time of test group was shorter than the control group, the blood recovery rate was higher than that of the control group, and the percentage of hemolysis in the 24 hours was significantly lower than that in the control group (P<0.05). Conclusion: Both the conventional method and the humid filter disk method can achieve the standard of leukocyte blood, but the wet filter disc method can reduce the filtration time, increase the blood recovery rate and reduce the blood disqualification rate caused by the hemolysis of leucocyte suspended red cell, which is worthy of clinical application. |
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