| 刘彩芳,孙妮娜,石 淼,陈 升,韩 川,吴 琼,倪 阵,袁 挺,张 剑,陆文全,时永全.外周血miR-17-92基因簇对于早期胃癌的诊断及预测价值[J].,2019,19(9):1653-1659 |
| 外周血miR-17-92基因簇对于早期胃癌的诊断及预测价值 |
| Diagnostic and Predictive Value of miR-17-92 Cluster in Peripheral Blood of Early Gastric Cancer |
| 投稿时间：2019-01-02 修订日期：2019-01-24 |
| DOI：10.13241/j.cnki.pmb.2019.09.011 |
| 中文关键词: 早期胃癌 miR-17-92基因簇 血清 诊断 预测 |
| 英文关键词: Early gastric cancer miR-17-92 cluster Serum Diagnosis Prediction |
| 基金项目:国家自然科学基金项目(81470805，81873554，2018TD-003) |
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| 中文摘要: |
| 摘要 目的：探讨外周血miR-17-92 簇对早期胃癌的诊断价值，为胃癌的早期诊断及治疗提供参考依据。方法：收集胃癌125例(Ⅰ期35例，Ⅱ期28例，Ⅲ期39例，Ⅳ期23例)和癌前病变24例(包括肠化生及上皮内瘤变)，同时选择65例慢性胃炎作为对照组。采用实时荧光定量PCR技术(Real-time quantitative PCR，RT-qPCR) 检测患者血清中的miR-17-92基因簇的表达水平。通过受试者工作曲线(Receiver Operating Curve, ROC)及曲线下的面积(Area Under the Curve，AUC)评估miR-17-92基因簇表达水平诊断早期胃癌的敏感性和特异性。结果：(1)慢性胃炎与癌前病变miR-17-92基因簇表达比较无显著差异(P>0.05)；(2)早期胃癌及进展期胃癌miR-17-5p表达明显高于慢性胃炎(P＜0.05)，miR-19a-3p、miR-19b-3p、miR-20a-5p和miR-92a-3p表达则显著低于慢性胃炎及进展期胃癌(P<0.05)；(3)miR-17-5p诊断早期胃癌的曲线下面积较miR-19a-3p、miR-19b-3p、miR-20a-5p、miR-92a-3p及CEA更高；(4)miR-19a-3p、miR-19b-3p、miR-20a-5p、miR-92a-3p高低表达组与在胃癌的浸润深度间有显著性差异(P<0.05)，miR-19b-3p高低表达组在胃癌的临床分期间有显著性差异(P<0.05)；(5)miR-17-5p、miR-19a-3p、miR-19b-p、miR-20a-5p、miR-92a-3p诊断早期胃癌的阳性率较CEA、CA199高。结论：外周血miR-17-92基因簇对于早期胃癌的诊断价值明显优于CEA和CA199，这可能为胃癌的早诊早治提供新的策略。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the diagnostic value of miR-17-92 cluster in the peripheral blood of early gastric cancer and pro- vide references for the early diagnosis and treatment of gastric cancer. Methods: A total of 125 cases with gastric cancer(stage I, 35 cases, stage II, 28 cases, stage III, 39 cases, stage IV, 23 cases) and 24 cases with precancerous lesion(including intestinal metaplasia and in- traepithelial neoplasia)were collected in this experiment. Meanwhile, 65 cases with chronic gastritis was selected as the control group.Real-time quantitative PCR (RT-qPCR) was used to detect the expression of miR-17-92 cluster in the serum. The receiver operating curve (ROC) and area under the curve (AUC) were used to assess the sensitivity and specificity of the expression of miR-17-92 cluster in the diagnosis of early gastric cancer. Results: (1)The expression of miR-17-92 cluster was not significantly different between chronic gas- tritis and precancerous lesion(P>0.05); (2)The expression of miR-17-5p in early gastric cancer and advanced gastric cancer was higher than that of chronic gastritis(P<0.05). The level of miR-19a-3p, miR-19b-3p, miR-20a-5p and miR-92a-3p were significantly lower than those of chronic gastritis and advanced gastric cancer(P<0.05); (3)The AUC of miR-17-5p is higher than miR-19a-3p, miR-19b-3p, miR-20a-5p and miR-92a-3p in early gastric cancer; (4)High and low expression of miR-19a-3p, miR-19b-3p, miR-20a-5p, miR-92a-3p is associated with invasive depth of gastric cancer(P<0.05) and low expression of miR-19b-3p was related to clinical stage of gastric can- cer(P<0.05); (5)The positive rate of miR-17-5p, miR-19a-3p, miR-19b-3p, miR-20a-5p, miR-92a-3p are higher than CEA and CA199 in the diagnosis of early gastric cancer. Conclusion: The diagnostic value of miR-17-92 cluster in the peripheral blood is better than CEA and CA199 for the early gastric cancer, which may provide a new strategy for the early diagnosis and treatment of gastric cancer. |
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