| 徐艳春,冯婕妤,胡毅倩,朱茂林,朱 煌.900 MHz手机辐射对人视网膜色素上皮细胞增殖活性及转化生长因子β2表达的影响[J].,2019,19(9):1608-1612 |
| 900 MHz手机辐射对人视网膜色素上皮细胞增殖活性及转化生长因子β2表达的影响 |
| Effect of 900 MHz Mobile Phone Radiation on Proliferative Activity and Expression of Transforming Growth Factor-β2 in Human Retinal Pigment Epithelium |
| 投稿时间：2018-09-27 修订日期：2018-10-23 |
| DOI：10.13241/j.cnki.pmb.2019.09.002 |
| 中文关键词: 手机 辐射 人视网膜色素上皮细胞 转化生长因子β2 眼部疾病 |
| 英文关键词: Mobile Radiation Human retinal pigment epithelium Transforming growths factor-β2 Ocular disease |
| 基金项目:上海市科委基金项目(17411950200&17411950206)；上海市卫生局中医药科研基金项目(2014JP015A) |
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| 中文摘要: |
| 摘要 目的：研究900 MHz手机辐射对人视网膜色素上皮细胞(RPE)增殖活性及转化生长因子β2(TGF-β2)表达的影响。方法：采用体外培养的RPE细胞，给予900 MHz手机电磁辐射者处理作为辐射组，未给予辐射者作为对照组。通过CCK8法检测RPE细胞的增殖活性，Real-Time PCR法检测RPE细胞TGF-β2 mRNA的表达，ELISA法检测RPE细胞培养上清液中TGF-β2的蛋白含量。结果：与对照组相比，辐射组RPE细胞的增殖活性降低，TGF-β2 mRNA表达增加，RPE细胞培养上清中TGF-β2含量上调，且差异具有统计学意义(P<0.05)。结论：900 MHz的手机辐射可能能通过调节TGF-β2的表达和释放导致RPE细胞增殖活性降低相关的眼部疾病。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of 900 MHz mobile phone radiation on the proliferative activity and expression of transforming growth factor-β2(TGF-β2) in human retinal pigment epithelium (RPE). Methods: RPE cells were cultured in vitro and di- vided into two groups. The radiation group was given exposure with a 900 MHz mobile phone in call and the control group was not given any exposure. The proliferation of RPE cells was detected by the cell-counting kit-8 (CCK8) assay. The expression of TGF-β2 mRNA and protein secretion of RPE cells were measured by Real-Time quantitative polymerase chain reaction (Real-Time PCR) and Enzyme- linked immunosorbent assay (ELISA) respectively. Results: The viability of RPE cells in radiation group was lower compared to that of the control group. The level of TGF-β2 mRNA and secretion were up-regulated under the exposure in contrast with the control group(P<0.05). Conclusion: 900 MHz mobile phone radiation may have an underlying association with some eye diseases related with de- creased proliferative activity of RPE cells by regulating the expression and release of TGF-β2. |
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