| 刘凤霞,艾孜孜·热合曼,斯依提·阿木提,刘文娟,蒋 萍,阿地力江·伊明.降钙素基因相关肽、血管活性肠肽在复合应激大鼠模型阴茎组织中表达及伊木萨克干预的研究[J].,2019,19(8):1446-1449 |
| 降钙素基因相关肽、血管活性肠肽在复合应激大鼠模型阴茎组织中表达及伊木萨克干预的研究 |
| Calcitonin Gene Related Peptide and Vasoactive Intestinal Peptide Expression in Penis Tissue of Complex Stress Rats Model and the Yimusake Intervention Effect |
| 投稿时间：2018-10-03 修订日期：2018-10-27 |
| DOI：10.13241/j.cnki.pmb.2019.08.010 |
| 中文关键词: 勃起功能障碍 降钙素基因相关肽 血管活性肠肽 伊木萨克 |
| 英文关键词: Erectile dysfunction Calcitonin gene related peptide Vasoactive intestinal peptide Yimusake |
| 基金项目:国家自然科学基金联合基金项目（U1303322，81860781）；新疆维吾尔自治区“十三五”重点学科（2050205） |
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| 中文摘要: |
| 摘要 目的：检测复合应激大鼠模型阴茎组织中降钙素基因相关肽（CGRP）、血管活性肠肽（VIP）的表达，并观察伊木萨克片对二者表达的影响。方法：选用56只正常雄性SD大鼠，其中10只为正常对照组（N），余46只为造模组，采用富含环境雌激素饲料+寒冷环境的干预条件建立复合性应激大鼠模型（20 w），并随机将其分为模型组（B1）、自然恢复组（B2）和伊木萨克干预组（B3），药物干预2 w后，免疫组化及Western blot方法检测大鼠阴茎组织中CGRP、VIP的表达。结果：①大鼠阴茎组织中CGRP表达：B1、B2组较N组明显减少（P＜0.05）；B3组较B1、B2组明显增多（P＜0.05）。②大鼠阴茎组织中VIP表达：B1、B2组较N组显著降低（P＜0.05）；B3组较B1、B2组显著升高（P＜0.05）。结论：复合应激大鼠模型阴茎组织中CGRP、VIP明显减少，伊木萨克片干预可抑制此变化。 |
| 英文摘要: |
| ABSTRACT Objective: To detect the expression of CGRP、VIP in the penis tissue of complex stress rats model, and explore the role of Yimusake. Methods: A total of 56 mature male Sprague-Dawley rats were randomly divided into 2 groups: normal control group (N, n = 10) that had normal environment and diet, and model group (n = 46) were treated with spinach and coriander diet in a cold-wet environment for 24 w. Then, the model rats were randomly divided into 3 groups (B1, B2, B3), after treated by Yimusake (250 mg/kg) for 2 weeks, CGRP, VIP were detected by immunohistochemistry (IHC) and Western-blot analysis. Results: ①CGRP expression in rat penis tissue: B1, B2 groups were decreased compared with N group(P＜0.05); B3 group was increased compared with B1, B2 group (P＜0.05). ②VIP expression in rat penis tissue: B1, B2 groups were decreased compared with N group(P＜0.05); B3 group was increased compared with B1, B2 group (P＜0.05). Conclusion: CGRP and VIP expression were decreased in the complex stress rats model, Yimusake can promote the CGRP and VIP expression. |
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