徐自慧,王雅蕾,万亮琴,何 芳,陈紫微,王 雪,宋晨晨,谭 琰,王 旭,董瑞娟,葛东宇,华 茜.肝癌小鼠外周血及脾脏T淋巴细胞亚群的变化及其意义[J].,2019,19(8):1406-1409 |
肝癌小鼠外周血及脾脏T淋巴细胞亚群的变化及其意义 |
The Changes of T Cell Subsets in Peripheral Blood and Spleen and Its Significance in Hepatoma Mice |
投稿时间:2018-08-08 修订日期:2018-08-31 |
DOI:10.13241/j.cnki.pmb.2019.08.002 |
中文关键词: 小鼠原位肝癌模型 T淋巴细胞亚群 流式细胞术 |
英文关键词: Orthotopic liver cancer model T cell subsets Flow cytometry |
基金项目:国家自然科学基金项目(81473546);藏医药区域协同创新中心项目(2017XTCX012);中央高校基本科研业务费专项基金资助(2018-JYBZZ-XJSJJ011) |
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中文摘要: |
摘要 目的:观察小鼠原位肝癌模型外周血以及脾脏T淋巴细胞亚群与正常小鼠之间的差异变化,探讨其差异变化的意义。方法:在正常KM小鼠肝脏种植H22细胞,建立小鼠原位模型。采用流式细胞术,以健康正常小鼠为对照,检测肝癌小鼠外周血以及脾脏T淋巴细胞亚群的变化。结果:与健康正常小鼠相比,肝癌小鼠外周血CD4+T淋巴细胞、CD4+/CD8+比例有显著性降低,CD8+T淋巴细胞显著性升高;脾脏CD3+、CD4++T淋巴细胞有显著性降低。结论:小鼠原位肝癌模型外周血以及脾脏T淋巴细胞亚群发生异常,免疫系统紊乱,可以反映小鼠肝癌的发生、发展。 |
英文摘要: |
ABSTRACT Objective: To observe the difference of T cell subsets in peripheral blood and spleen between normal mice and orthotopic liver cancer model, and to explore the significance of the difference. Methods: H22 cells were implanted in the liver of KM mice to establish hepatoma mice and flow cytometry was used to test the changes of T cell subsets in peripheral blood and spleen in hepatoma mice and normal mice as control. Results: Compared with normal mice, the proportion of CD4+ T cells and CD4+/CD8+ in peripheral blood of hepatoma mice was significantly decreased, well, the proportion of CD8+ T cells significantly increased. The proportion of CD3+, CD4++ T cells in spleen was significantly reduced. Conclusion: After H22 cells were implanted in the liver of mice, the T cell subsets in peripheral blood and spleen were abnormal, and the immune system was disordered. It can reflect the occurrence and development of liver cancer in mice. |
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