| 蒋雪霞,尹道英,李晓玲,杨 艳,王博伟.Musashi-1和β-catenin在子宫内膜中的表达及其在内膜异位病灶形成中的作用[J].,2019,19(6):1138-1142 |
| Musashi-1和β-catenin在子宫内膜中的表达及其在内膜异位病灶形成中的作用 |
| Expression of Musashi-1 and β-catenin in Endometrium and Its Role in the Formation of Endometriosis Lesions |
| 投稿时间：2018-09-12 修订日期：2018-09-30 |
| DOI：10.13241/j.cnki.pmb.2019.06.030 |
| 中文关键词: 子宫内膜异位症 Musashi-1 β-连环蛋白 作用机制 |
| 英文关键词: Endometriosis Musashi-1 β-catenin Mechanism of action |
| 基金项目:重庆市卫生局科研基金项目（CW2014B136） |
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| 中文摘要: |
| 摘要 目的：检测Musashi-1和?茁-连环蛋白（β-catenin）在子宫内膜异位症（EMs）患者的在位内膜和异位内膜中的表达，并初步探讨作用机制。方法：2016年9月至2018年9月，收集EMs患者的在位内膜（在位内膜组，28例）、异位内膜（异位内膜组，24例）和非EMs患者的正常内膜（正常内膜组，30例），采用实时荧光定量PCR（Real-time PCR）试验检测Musashi-1和β-catenin在各组内膜组织中的表达情况，并分析Musashi-1和β-catenin在各组内膜组织中的表达相关关系。结果：在位内膜组和异位内膜组中，Musashi-1和?茁-catenin的相对表达量均明显高于正常内膜组（P<0.05），而在位内膜组与异位内膜组之间的差异无统计学意义（P>0.05）。在位内膜组和正常内膜组中，增生期的Musashi-1和β-catenin相对表达量显著高于分泌期（P<0.05），而异位内膜组中，在增生期和分泌期Musashi-1和β-catenin比较差异无统计学意义（P>0.05）。在位内膜组和异位内膜组中，Musashi-1和β-catenin表达之间均呈正相关性（P<0.05），而正常内膜组中，两者表达之间无明显相关性（P>0.05）。结论：在EMs的发病过程中，干细胞标志物Musashi-1可能通过激活Wnt/β-catenin信号通路参与并促进子宫内膜异位病灶的形成。 |
| 英文摘要: |
| ABSTRACT Objective: To detect the expressions of Musashi-1 and β-catenin in the eutopic endometrium and ectopic endometrium of patients with endometriosis (EMs), and to preliminarily explore the possible mechanism. Methods: Eutopic endometrium of patients with EMs (eutopic endometrium group, 28 cases), ectopic endometrium of patients with EMs (ectopic endometrium group, 24 cases) and normal endometrium of people without EMs (normal endometrium group, 30 cases) were collected during September 2016 to September 2018. The expression of Musashi-1 and β-catenin in the endometrium tissue of each group was detected by real time fluorescence quantitative PCR (Real-time PCR) test, and the correlation between the expression of Musashi-1 and β-catenin in the endometrium tissue of each group was analyzed. Results: The relative expression quantity of Musashi-1 and β-catenin in the eutopic endometrium group and the ectopic endometrium group was significantly higher than those in the normal endometrium group (P<0.05), while there was no difference between the eutopic endometrium group and the ectopic endometrium group (P>0.05). In the eutopic endometrium group and the normal endometrium group, the relative expression quantity of Musashi-1 and β-catenin in the proliferative period was significantly higher than those in the secretory period (P<0.05), but there was no difference between the proliferative period and the secretory period in the ectopic endometrium group (P>0.05). There was a positive correlation between the expression of Musashi-1 and β-catenin in the eutopic endometrium group and the ectopic endometrium group (P<0.05), but there was no correlation between the expression of Musashi-1 and β-catenin in the normal endometrium group (P>0.05). Conclusion: In the pathogenesis of EMs, stem cell marker Musashi-1 may participate in and promote the formation of endometriosis lesions by activating Wnt/β-catenin signaling pathway. |
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