| 李加德,白 璐,李 琦,王新玲,曹孟儒,黄小义.5AZA-CdR 影响UPP1在结肠癌中的表达[J].,2019,19(6):1024-1032 |
| 5AZA-CdR 影响UPP1在结肠癌中的表达 |
| 5AZA-CdR Affects the Expression of UPP1 in Colon Cancer |
| 投稿时间：2018-09-05 修订日期：2018-09-30 |
| DOI：10.13241/j.cnki.pmb.2019.06.006 |
| 中文关键词: UPP1 5AZA-CdR 恶性肿瘤 预后 |
| 英文关键词: UPP1 5AZA-CdR Pan-cancer Prognosis |
| 基金项目:黑龙江省留学归国人员科学基金项目（LC2016037） |
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| 中文摘要: |
| 摘要 目的：探讨5-氮杂-2'-脱氧胞嘧啶核（5AZA-CdR）对尿苷磷酸化酶（uridine phosphorylase，UPP1）在结肠癌中表达的影响以及UPP1在多种恶性肿瘤中的表达及其与患者临床预后的相关性。 方法：用5AZA-CdR处理结肠癌细胞系并进行裸鼠成瘤实验检测UPP1的表达情况。在TCGA数据库中对UPP1在多种肿瘤中的表达情况进行分析。使用在线数据库GEPIA评估UPP1 mRNA表达对患者预后影响。使用cBioPortal在线软件在10945个肿瘤样本中对UPP1参与的调控网络进行预测。 结果：5AZA-CdR明显抑制结肠癌细胞系SW620以及SW1116的增殖（P<0.05）； 用5AZA-CdR对结肠癌细胞系进行处理后UPP1表达量显著增加（P<0.05）。体内实验结果显示5AZA-CdR处理后肿瘤重量显著降低；在裸鼠模型中，5AZA-CdR处理后UPP1表达量增加（P<0.05）。通过比对23种肿瘤细胞与其相对应的正常细胞的表达谱数据，结果显示在大多数肿瘤中UPP1呈高表达状态。使用在线数据库GEPIA对肿瘤患者的生存率进行了全面的研究结果显示UPP1表达高患者的总生存率差和无病生存率均较差。通过cBioPortal在线软件对UPP1参与的调控网络进行预测，UPP1能够参与多种基因的表达调控。结论：5AZA-CdR 抑制肿瘤生长并增加UPP1的表达；UPP1高表达与患者的预后不良相关，因此UPP1可能成为一种潜在的肿瘤诊断和预后因子。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of 5AZA-CdR on the expression of uridine phosphorylase (UPP1) in colon cancer and UPP1 expression in various malignant tumors and also its correlation with clinical prognosis of patients. Methods: Detection of UPP1 expression in both colon-cancer cells and nude mice bearing tumor subcutaneously treated with 5AZA-CdR. Analysis of UPP1 expression in various tumors in TCGA database. Evaluation of the effect of UPP1 expression on patient prognosis by database GEPIA. Prediction of the UPP1 involved regulatory network in 10945 tumor samples with cBioPortal. Results: 5AZA-CdR significantly inhibited the proliferation of SW620 and SW1116 colon cancer cell lines （P < 0.05）, UPP1 expression was significantly increased in colon cancer cell lines treated with 5AZA-CdR (P < 0.05). In vivo, the tumor weight was significantly decreased after 5AZA-CdR treatment and the expression of UPP1 was increased after 5AZA-CdR treatment in the nude mouse model (P < 0.05). UPP1 was overexpressed in most tumors by comparising the expression profile of 23 types of tumor cells and normal cells. UPP1 high-expressed patients had poor overall survival and disease-free survival by analyzing tumor patients survival on GEPIA database. UPP1 could participate in the regulation of multiple gene expression by predicting its involved in regulatory network on cBioPortal soft. Conclusion: 5AZA-CdR inhibits tumor growth and increases UPP1 expression; the high-expression of UPP1 is closely associated with poor prognosis, thus UPP1 possibly becomes a novel tumor marker and the target for tumor theray. |
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