| 黄晓燕,郑超伟,罗莉川,赵一娜,王 萌,刘熙荣,李生发.四君子汤对溃疡性结肠炎小鼠模型occludin和claudin-1表达的影响[J].,2019,19(5):829-833 |
| 四君子汤对溃疡性结肠炎小鼠模型occludin和claudin-1表达的影响 |
| Effect of Sijunzi Decoction on Occludin and Claudin-1 Expression in Ulcerative Colitis Mice Model |
| 投稿时间：2018-10-06 修订日期：2018-10-30 |
| DOI：10.13241/j.cnki.pmb.2019.05.007 |
| 中文关键词: 四君子汤 溃疡性结肠炎 紧密连接蛋白 occludin claudin-1 |
| 英文关键词: Sijunzi decoction Ulcerative colitis Tight junction protein occludin claudin-1 |
| 基金项目:国家自然科学基金项目（81460714）；广西壮族自治区自然科学基金项目（2014GXNSFBA118183） |
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| 中文摘要: |
| 摘要 目的：探讨四君子汤对溃疡性结肠炎（UC）小鼠模型结肠粘膜中occludin、claudin-1表达的影响。方法：采用右旋葡聚糖硫酸钠(DSS)诱导UC小鼠模型，实验分为五组，即正常组、模型组、四君子汤低剂量治疗组、中剂量治疗组、高剂量治疗组、西药组，共治疗7天。对小鼠肠黏膜的大体形态和组织病理变化进行观察，使用RT-PCR和Western blot检测occludin、claudin-1 mRNA和蛋白的表达。结果：与模型组相比较，四君子汤低、中、高剂量治疗组以及西药组小鼠的饮食、体重、精神、活动度、脓血便等一般情况有所改善，黏膜层缺损、隐窝破坏、炎症细胞浸润等病理表现有所缓解。与模型组相比较，高剂量治疗组小鼠结肠组织中occludin、claudin-1蛋白和mRNA的表达升高(P＜0.05)，低剂量和中剂量治疗组也有不同程度的升高。与西药组相比较，低、中、高剂量治疗组小鼠结肠组织中occludin、claudin-1蛋白和mRNA的表达无统计学差异(P＞0.05)。结论：四君子汤可以改善脓血便等症状，缓解肠粘膜的损伤，上调occludin和claudin-1的表达，对UC小鼠有治疗作用。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of Sijunzi decoction on occludin and claudin-1 expression in ulcerative colitis(UC) mice model. Methods: The UC mice model was induced by dextran sulfate sodium(DSS). The experiment was divided into five groups: normal group, model group, low-dose treatment group, middle-dose treatment group, high-dose treatment group and western medicine group. The treatment lasted for 7 days. The morphological and histopathological changes of intestinal mucosa in mice were observed. The mRNA and protein expressions of occludin and claudin-1 were detected by RT-PCR and Western blot. Results: Compared with the model group, the diet, body weight, spirit, activity and bloody purulent stool of the mice in the low, middle and high dose Sijunzi decoc- tion treatment group and Western medicine group were improved, and the pathological manifestations such as mucosal defect, crypt de- struction and inflammatory cell infiltration were alleviated. Compared with the model group, the protein and mRNA expressions of oc- cludin and claudin-1 in the high-dose treatment group increased(P<0.05), and the protein and mRNA expressions of occludin and claudin-1 in the low-dose and middle-dose treatment groups also increased in varying degrees. Compared with the western medicine group, there was no significant difference in the protein and mRNA expressions of occludin and claudin-1 in the low, middle and high dose groups (P>0.05). Conclusion: Sijunzi Decoction can improve the symptoms of bloody purulent stool, relieve the damage of intestinal mucosa, up-regulate the expression of occludin and claudin-1, and has therapeutic effect on UC mice. |
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