| 朱 珊,黄亚楷,张伟民,董 丹,闫俊强.APP/PS1年轻小鼠的早期学习记忆缺陷及血清注射治疗效果分析[J].,2019,19(5):811-815 |
| APP/PS1年轻小鼠的早期学习记忆缺陷及血清注射治疗效果分析 |
| Serum Injection Rescues Learning and Memory Deficits in APP/PS1 Young Mice |
| 投稿时间：2018-08-23 修订日期：2018-09-18 |
| DOI：10.13241/j.cnki.pmb.2019.05.003 |
| 中文关键词: 阿尔兹海默症 APP/PS1 认知能力 学习记忆 血清 |
| 英文关键词: Alzheimer's Disease APP/PS1 Recognition Learning and memory Serum |
| 基金项目:国家自然科学基金项目(U1304809) |
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| 中文摘要: |
| 摘要 目的：明确经典阿尔兹海默症(Alzheimer's Disease，AD)小鼠模型APP/PS1的年轻小鼠是否存在学习记忆障碍，并探讨尾静脉注射同龄小鼠的血清是否可以改善年老AD小鼠的认知能力。方法：根据转基因小鼠的基因型，将同龄小鼠分为wildtype(WT)和APP/PS1两组，首先用物体辨别实验(Novel object recognition，NOR)检测2个月龄小鼠的认知能力(90min retention: WT n=6, APP/PS1 n=8; 24hours retention: WT n=7, APP/PS1=8)，同时用Morris水迷宫实验(Morris water maze，MWM)检测2个月龄小鼠的空间学习记忆能力(WT n=6, APP/PS1 n=5)；采用内眦取血法从8月龄小鼠中获取全血，高速离心获得血清。将8月龄APP/PS1小鼠分为两组：对照组注射PBS(n=7)，实验组注射血清(n=6)，每周注射两次，100 μL/只/次，连续注射3周。注射结束后，用NOR法检测对照组和实验组小鼠的认知能力。结果：NOR实验结果显示APP/PS1小鼠的辨别指数(Discrimination index(%))显著低于WT小鼠(P<0.05)；MWM实验结果显示APP/PS1小鼠到达平台的时间明显长于WT小鼠，同时在测试阶段中，APP/PS1小鼠在目的象限的探索时间及穿越次数显著低于WT小鼠(P<0.05)；治疗实验中，与对照组APP/PS1小鼠的辨别指数相比较，实验组APP/PS1小鼠在注射同龄小鼠的血清后，其物体辨别指数显著升高(P<0.05)，小鼠脑中的Aβ沉淀明显减少。结论：APP/PS1小鼠在2个月左右就会表现出明显的学习记忆障碍；注射正常同龄鼠的血清可以明显改善APP/PS1小鼠的学习记忆能力同时阻碍Aβ沉淀的形成。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the learning and memory ability in young Alzheimer's disease (AD) APP/PS1 mouse model, and identify whether serum injection can rescue these defects. Methods: According to the genotype, the same age male mice were divided into two groups, the wildtype (WT) and APP/PS1 groups. Firstly, we performed novel object recognition (NOR) to evaluate the recogni- tion ability of two-months old male mice (90 min retention: WT n=6, APP/PS1 n=8; 24hours retention: WT n=7, APP/PS1=8), and carried out Morris water maze (MWM) to test the spatial learning and memory in two-months old mice (WT n=6, APP/PS1 n=5); Blood was col- lected from the inner canthus of 8-months old mice, and the serum was obtained by super-centrifugation. 8-months old APP/PS1 mice were divided into two groups, the control group which was injected by PBS buffer (APP/PS1 n=7), and the experimental group was in- jected with serum (APP/PS1 n=6), injection was performed twice a week, 100 μL/mice/time, for 3 weeks. After injection, NOR was per- formed to evaluate the therapeutic effect. Results: Compared with the WT mice, the discrimination index of APP/PS1 mice is significantly decreased in NOR (P<0.05). The latency of APP/PS1 mice is obviously longer than that of WT mice (P<0.05). Moreover, compared with those of WT mice, the time spent and crossing number of APP/PS1 in target quadrant are significantly reduced during the testing stage (P<0.05). About the rescue experiment, we find that the group which injected with serum obtained higher discrimination index and less Aβ plaque than those in PBS injected group(P<0.05). Conclusion: Obvious learning and memory deficits occurred in APP/PS1 young mice at about 2-months old, and the same-age serum injection could rescue recognition defects and reduce Aβ formation in APP/PS1 mice. |
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