| 张丽娜,肖东方,董海龙,武 婷,王 智.Orexin能神经元在依托咪脂麻醉中的促觉醒作用[J].,2019,19(5):807-810 |
| Orexin能神经元在依托咪脂麻醉中的促觉醒作用 |
| The Role of Orexinergic Neurons in the Etomidate Anesthesia |
| 投稿时间：2018-07-28 修订日期：2018-08-23 |
| DOI：10.13241/j.cnki.pmb.2019.05.002 |
| 中文关键词: Orexin 全身麻醉 依托咪脂 |
| 英文关键词: Orexin General Anethesia Etomidate |
| 基金项目:国家自然科学基金面上项目(81571351)；国家自然科学基金青年基金项目(81701362)；陕西省重点研发计划——一般项目社会发展领域(2018SF-057) |
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| 中文摘要: |
| 摘要 目的：观察orexin能神经元在依托咪脂麻醉中的促觉醒作用。方法：选择雄性SD大鼠36只，体重230～250g。将18只SD大鼠随机分为脂肪乳剂组(60 mg·kg-1·h-1)，依托咪脂麻醉(50 mg·kg-1·h-1)30 min组和60 min组(每组n=6)，用放射免疫法检测3组大鼠血浆中orexin含量；3天后，将脂肪乳剂组大鼠颈椎脱臼处死后灌注取脑，另外两组在依托咪脂麻醉下灌注取脑，采用免疫荧光双标染色，分别观察3组大鼠orexin神经元活性。另取18只SD大鼠随机分为乳酸林格氏液组，orexin-A 30 pmol组和100 pmol 组(每组n=6)，记录大鼠翻正反射消失时间为麻醉诱导时间及翻正反射恢复时间为觉醒时间。结果：与脂肪乳剂组相比，依托咪脂麻醉30 min和60 min后，血浆orexin-A含量下降(P<0.05)，有活性的orexin神经元数目减少(P<0.01)；与依托咪脂麻醉30 min组相比，60 min组有活性的orexin神经元数目减少(P<0.05)，但血浆orexin-A含量与30 min组无差异(P>0.05)；与乳酸林格氏溶液组相比，基底前脑区微注射orexin-A 30 pmol或100 pmol对麻醉诱导无影响，但能显著缩短依托咪脂麻醉觉醒时间(P<0.05, P<0.01)；但orexin-A 30 pmol组和100 pmol组诱导和觉醒时间比较均无统计学差异(P>0.05)。结论：依托咪脂麻醉抑制大鼠下丘脑orexin神经元的活性，orexin-A对依托咪脂麻醉具有促觉醒作用。 |
| 英文摘要: |
| ABSTRACT Objective: To evaluate the stimulative effect of orexinergic neurons on the etomidate anesthesia. Methods: Thirty-six male SD rats weighed 230～250 g were used in this study. Eighteen SD rats were randomly divided into the emulsion group (60 mg·kg-1·h-1), the etomidate pumping (50 mg·kg-1·h-1) 30min and 60min group (n=6 each). Blood was firstly taken from the femoral vein to deter- mine the concentrations of orexin-A in plasma by radioimmunoassay. Three days later, the rats in emulsion groupwere killed by cervical dislocation and then infused by polyoxymethylene, the other two groups were pumped etomidate and infused by polyoxymethylene at 30 min and 60 min respectively, then immunofluorescence was used to detect the activation of orexinergic neurons before and after etomi- date anesthesia. The other eighteen SD rats were randomly divided into the Ringer's group, orexin-A 30 pmol group and 100 pmol group (n=6 each). The loss of righting reflex, induction time and the return of righting reflex which was recorded as emergence time were com- pared between different groups. Results: Compared with the emulsion group, the concentration of orexin-A in plasma was significantly decreased(P<0.05) and the number of activated orexinergic nureons were also decreased in etomidate 30 min and 60 min group (P<0.01); in contrast to etomidate 30min group, the number of activated orexinergic nureons decreased in etomidate 60 min group but the concen- tration of orexin-A in plasma had no change(P>0.05). In contrast to ringer's group, microinjection of 30 or 100 pmol orexin-A in basal forebrain had no effect on induction time but significantly shorted the emergence time of etomidate anesthesia (P<0.05, P<0.01); they was no significant difference in the induction and emergence time between orexin-A 30 pmol group and 100 pmol group(P>0.05). Conclusion: The activation of orexinergic neurons was depressed by etomidate anesthesia and orexin-A could promote the emergence time of etomi- date. |
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