| 陈宝艳,甄玉花,赵 颖,王淑平,孙 雪.疏肝健脾法对产后缺乳大鼠催乳素及其受体表达的影响研究[J].,2019,19(4):620-625 |
| 疏肝健脾法对产后缺乳大鼠催乳素及其受体表达的影响研究 |
| Research on the Effect of Shugan Jianpi Method on the Morphology, PRL and PRLR in Postpartum Hypogalactia Rats |
| 投稿时间：2018-08-10 修订日期：2018-09-06 |
| DOI：10.13241/j.cnki.pmb.2019.04.004 |
| 中文关键词: 疏肝健脾法 产后缺乳 大鼠 乳腺组织 PRL |
| 英文关键词: Shugan Jianpi method Postpartum hypogalactia Rats Breast tissue PRL |
| 基金项目:国家自然科学基金项目（81603653） |
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| 中文摘要: |
| 摘要 目的：观察疏肝健脾法对产后缺乳肝郁脾虚证大鼠乳腺组织形态学与催乳素（prolactin，PRL）及其受体（prolactin receptor，PRLR）表达的影响。方法：采用甲磺酸溴隐亭溶液+慢性束缚法复制大鼠产后缺乳肝郁脾虚病证结合动物模型，随机分为模型组、空白对照组、西药对照组、中药低、中、高剂量组，每组8只，共6组。药物干预后，采用HE染色及免疫组化法比较不同干预方法对产后缺乳大鼠乳腺组织形态与催乳素及其受体表达的影响，匀浆乳腺组织，采用ELISA法检测乳腺组织催乳素（PRL）、血清催乳素（PRL）、胃泌素（GAS）、血清下丘脑一垂体一肾上腺轴（The hypothalamic-pituitary-adrenal axis，HPA 轴）水平变化：促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）、皮质醇（COR）。结果：与模型组相比，西药对照组和中药低、中、高剂量组均可促进乳腺组织向泌乳活跃期发育，表现为腺泡腔充盈、腺泡上皮细胞数量增多、结缔组织和脂肪组织退化，同时增加乳腺组织中PRL（空白对照组（145.72±16.332）、中药中剂量组（127.39±10.854）＞西药对照组（80.79±18.112）、中药低剂量组（89.42±14.183）、中药高剂量组（88.02±12.064）＞模型组（54.32±7.128））（P＜0.05）及PRLR（空白对照组（139.76±14.199）、中药中剂量组（129.09±18.755）＞西药对照组（87.42±8.390）、中药低剂量组（81.54±11.090）、中药高剂量组（85.167±9.494）＞模型组（60.93±11.767））的表达（P＜0.05）。与模型组相比，疏肝健脾中药可降低过高的血清CRH、ACTH、COR水平，升高乳腺组织PRL、血清GAS及PRL水平（P＜0.05）。其中，中药中剂量组疗效优于其他用药各组（P＜0.05）。结论：疏肝健脾法促进产后缺乳大鼠的泌乳作用机制与增加PRL及其受体表达、调节胃肠功能、抑制HPA轴的亢进状态，从而促进乳腺组织形态向泌乳活跃期发育密切相关。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the effect of Shugan Jianpi methodon the histology, PRL and PRLR of mammary gland in rats with postpartum hypogalactia of liver stagnation and spleen deficiency syndrome. Methods: The rat model of postpartum hypogalactia of liver stagnationand spleen deficiency was reproduced by bromocriptine mesylate solution and chronic binding method. After the drug was gavaged, HE staining and immunohistochemistry were used to compare the effects of different interventions on mammary gland morphology and expression of prolactin and prolactin receptors in postpartum lactating rats. Serum and mammary gland PRL, serum GAS and the hypothalamic(The hypothalamic-pituitary-adrenal axis, HPA axis) related indicators were detected by enzyme-linked immuno sorbent assay(ELISA): serum corticotropin releasing hormone (CRH), serum adrenocorticotropic hormone(ACTH), serum cortisol (COR). Results: Compared to the model group, the methoxychloropylamine group and Chinese medicine of high, medium and low dose group can promote the development of breast tissue to active lactation, which is characterized by acinar cavity filling, increased number of acinar epithelial cells, and deterioration of connective tissue and adipose tissue. At the same time, the methoxychloropylamine group and Chinese medicine can increase the expression of PRL and PRLR in breast tissue. The effect of Chinese medicine medium dose treat- ment group is better than the other groups. In breast tissue, the relationship between PRL and PRLR is linearly related（r=0.845, P＜0.05）. Compared to the model group, serum COR, CRH and ACTH in the therapy group decreased significantly (P<0.05), while serum and mammary gland PRL and serum GAS level increased(P＜0.05). Conclusion: The mechanism of Shugan Jianpi method promoting lacta- tion in postpartum hypogalactia rats is closely related to increasing the expression of PRL and PRL receptor expression, regulating gas- trointestinal function, inhibiting hyperactivity of HPA axis, and thus promoting the development of breast tissue morphology to active lactation. |
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