文章摘要
吴 敏,陈俊梅,刘召波,刘海东,刘 超,张爱英,李 宁.乙型肝炎病毒核心抗体IgM的蛋白芯片检测法研究[J].,2019,19(3):560-562
乙型肝炎病毒核心抗体IgM的蛋白芯片检测法研究
Detection of Human Anti-hepatitis B Virus Core Antibody IgM by Protein Microarray
投稿时间:2018-05-22  修订日期:2018-06-18
DOI:10.13241/j.cnki.pmb.2019.03.038
中文关键词: 蛋白芯片  乙肝病毒  定性  抗HBc-IgM
英文关键词: Protein Microarray  Hepatitis B virus  Qualitative  Anti-HBc IgM
基金项目:北京市科技委员会基金项目(Z181100001018031)
作者单位E-mail
吴 敏 首都医科大学附属北京佑安医院 北京100069 wm13366621237@163.com 
陈俊梅 首都医科大学附属北京佑安医院 北京100069  
刘召波 首都医科大学附属北京佑安医院 北京100069  
刘海东 首都医科大学附属北京佑安医院 北京100069  
刘 超 首都医科大学附属北京佑安医院 北京100069  
张爱英 北京市肝病研究所 北京100069  
李 宁 1首都医科大学附属北京佑安医院 北京100069 2 北京市肝病研究所 北京100069  
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中文摘要:
      摘要 目的:探索蛋白芯片技术检测乙型肝炎病毒(HBV)抗HBc-IgM的可行性。方法:应用Nano-Plotter TM-压电式微量喷墨点阵制备系统自制的核心抗原蛋白芯片在经10%山羊血清封闭后加入待测血清37℃孵育,PBST清洗晾干后后再次加入检测抗体即HRP-抗人IgM抗体,芯片用伯乐成像仪检测有无信号。结果:24份HBsAg和抗HBc阳性血清中经蛋白芯片检测抗HBc-IgM阳性率为83.3%(20/24);24份健康志愿者血清中经蛋白芯片检测抗HBc-IgM阳性率为4.1%(1/24)。乙肝血清与健康志愿者血清化学发光信号差异明显。结论:蛋白芯片技术可较好地应用于定性检测HBV抗HBc-IgM,为临床快速判断是否存在HBV感染和监测慢性肝炎HBV活动性提供新的辅助诊断方法。
英文摘要:
      ABSTRACT Objective: To explore the feasibility of protein microarray for detecting human anti-hepatitis B virus core antibody IgM qualitatively. Methods: Protein microarray was fabricated by GeSiM Nano-PlotterTM Micropipetting System. The core antigen protein spotted on the slides with BSA spotted as control protein. All slides were blocked in a coupling buffer (10% normal goat serum with 0.1%NaN3). Then serum samples were added on the slides and incubated for 2h at 37℃. After rinsed by PBST, the slides were added with HRP-anti-human IgM antibody and then scanned by a chemiluminescent scanner, Chemi DocTM MP System (Bio-Rad, California, USA). Results: The positive rate of anti-HBc IgM was 83.3% (20/24) in 24 HBsAg and anti-HBc positive serum samples detected by protein microarray, while the positive rate of anti-HBc IgM was 4.1% (1/24) in 24 healthy volunteers. The chemiluminescent signal difference was significant between hepatitis B positive and healthy serum samples. Conclusion: Protein microarray technology could be applied to detect anti-HBc IgM qualitatively, and provide a new auxiliary diagnostic method for clinical rapid determination of HBV infection and monitoring HBV chronic hepatitis activity.
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