| 刘佐君,胡文静,孙世民,张树菊,钱民先.槲皮素对节律钟基因的调控研究*[J].,2019,19(1):47-51 |
| 槲皮素对节律钟基因的调控研究* |
| Effects of Quercetin on the Circadian Expression Pattern of Human Bone Osteosarcoma Epithelial U2OS Cells* |
| 投稿时间：2018-06-28 修订日期：2018-07-23 |
| DOI：10.13241/j.cnki.pmb.2019.01.010 |
| 中文关键词: 节律钟 槲皮素 氧化型烟酰胺腺嘌呤二核苷酸 组蛋白乙酰化 |
| 英文关键词: Circadian clock Quercetin Histone acetylation NAD+ |
| 基金项目:国家自然科学基金项目（81601215）；深圳市科技创新基础研究项目（JCYJ20150525092941002） |
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| 中文摘要: |
| 摘要 目的：探讨槲皮素对节律钟基因表达的影响。方法：通过50%的马血清刺激诱导人骨肉瘤U2OS细胞同步化，利用槲皮素处理同步化后的U2OS细胞。进一步利用荧光定量PCR检测节律钟关键基因的变化。利用western blot检测槲皮素对组蛋白乙酰化的影响，并且通过试剂盒测定槲皮素对细胞内氧化型烟酰胺腺嘌呤二核苷酸（NAD+）的影响。通过尼克酰胺和槲皮素同时处理U2OS细胞，利用荧光定量PCR检测节律钟关键基因的变化。结果：U2OS细胞经过槲皮素处理后节律钟关键基因芳烃受体核转位蛋白3（brain and muscle Arnt-like protein-1，BMAL1），节律周期蛋白2（Period 2, PER2），孤儿核受体alpha（REV-ERBα）和蓝光受体蛋白1（Cryptochrome 1，CRY1）在转录水平的表达水平有明显的升高。槲皮素处理可以显著降低U2OS细胞的组蛋白乙酰化的水平，并且显著升高U2OS细胞内的氧化型烟酰胺腺嘌呤二核苷酸的水平。进一步研究发现，尼克酰胺（Nicotinamide， NAM）处理完全抑制了槲皮素对节律基因的影响。结论：槲皮素显著地激活了节律钟关键基因的mRNA表达水平，槲皮素对于节律基因的调控依赖于Sirtuins的活性，其机制可能是由于槲皮素增加了细胞内的氧化型烟酰胺腺嘌呤二核苷酸的水平所导致。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of quercetin on the cell autonomous circadian rhythm of U2OS cells. Methods: The human U2OS cells were synchronized by 50% horse serum, and treated with DMSO or 200 μmol/L quercetin. The oscillation profiles of key circadian genes were examined by qRT-PCR. The histone acetylation levels were determined by western blot. The cellular levels of NAD+ were measured by NAD/NADH Quantitation Colorimetric Kit. U2OS cells were treated with Nicotinamide and quercetin. The expression of circadian genes were examined by qRT-PCR. Results: Quercetin significantly increased the amplitude of the mRNA levels of several key clock components, including BMAL1, CRY1, PER2 and REV-ERBα. Western blot analysis revealed quercetin treatment led to reduced histone acetylation levels. Quercetin dramatically increased cellular NAD+ levels. Moreover, the Sirtuins inhibitor, NAM, totally blocked the effects of quercetin on the expression of clock genes. Conclusion: Quercetin is a strong activator of circadian clock. The regulation of circadian genes expression by quercetin is totally dependent on Sirtuins activity, which might be due to elevated cellular NAD+ levels. |
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