| 吴 涛,丁志斌,许刚柱,盛旭东,卢文超,钟 海,张俊锋.创伤性脑损伤后IL-1?茁在神经胶质细胞中经时定位表达的研究*[J].,2019,19(1):43-46 |
| 创伤性脑损伤后IL-1?茁在神经胶质细胞中经时定位表达的研究* |
| Research of the Time Orientation Expression of IL-1?茁 in Glial Cellsafter Traumatic Brain Injury* |
| 投稿时间：2018-08-07 修订日期：2018-08-28 |
| DOI：10.13241/j.cnki.pmb.2019.01.009 |
| 中文关键词: 创伤性脑损伤 炎症因子 小胶质细胞 星形胶质细胞 表达 |
| 英文关键词: Traumatic brain injury Inflammatory factors Microglia Astrocytes Expression |
| 基金项目:陕西省社会发展科技攻关项目（2015FS0638） |
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| 中文摘要: |
| 摘要 目的：探讨创伤性脑损伤（TBI）后白细胞介素-1β（IL-1β）在神经胶质细胞中的经时定位表达情况。方法：选择72只SPF级雄性小鼠分为假手术组（sham组）、TBI 6h组、TBI 12h组、TBI 1d组、TBI 4d组与TBI 7d组，每组12只，分别在脑损伤后6h、12h、1d、4d、7d时获取血清和脑组织并且制作切片。ELISA检测损伤后炎症因子IL-1β、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达。Western blot检测钙离子结合蛋白-1（IBA-1）和胶质纤维酸性蛋白（GFAP）的表达。应用免疫荧光双重染色技术观察炎症因子IL-1β在小胶质细胞和星形胶质细胞中的定位表达情况。结果：TBI后6h-7d时炎症因子IL-1β、IL-6和TNF-?琢的表达量均高于sham组（P<0.05）。Western blot结果显示，IBA-1的表达在损伤后6h-7d时高于sham组，GFAP的表达在损伤后1d-7d时高于sham组（P<0.05）。免疫荧光双重染色技术显示，6h、12h时IL-1β主要表达在小胶质细胞中，IL-1β和IBA-1共表达细胞数量多于sham组（P<0.05）；1d、4d、7d时IL-1β主要表达在星形胶质细胞中，IL-1β和GFAP共表达细胞数量多于sham组（P<0.05）。结论：TBI诱导了胶质细胞和炎症因子的表达，其表达随脑损伤的时间而变化，IL-1β早期定位表达于小胶质细胞，后期定位表达于星形胶质细胞中。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the time orientation expression of interleukin-1β (IL-1β) in glial cells after traumatic brain injury (TBI). Methods: 72 cases of SPF male mice were selected and divided into sham operation group (group sham), group TBI 6h, group TBI 12h, group TBI 1d, group TBI 4d, group TBI 7d, 12 cases in each group. Serum and brain tissues were obtained after brain injury at 6h, 12h, 1d, 4d and 7d. The expression of IL-1β, interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were detected by ELISA.The expression of ionized calcium binding adaptor molecule-1(IBA-1) and glial fibrillary acidic protein (GFAP) were detected by Western blot. The expression of IL-1β in microglia and astrocytes was observed by immunofluorescence double staining. Results: The expression inflammatory factors IL-1β, IL-6, TNF-α were higher than sham group at 6h-7d after TBI (P<0.05). Western blot results showed that the expression of IBA-1 were higher than sham group at 6h-7d after injury, the expression of GFAP were higher than sham group at 1d-7d after injury (P<0.05). Immunofluorescence double staining showed that IL-1β was mainly expressed in microglia at 6h and 12h, the number of IL-1β and IBA-1 coexpressed were more than that in sham group (P<0.05). IL-1β was mainly expressed in astrocytes at 1d, 4d and 7d, the number of IL-1β and GFAP coexpressed were more than that in sham group (P<0.05). Conclusion: TBI induced the expression of glial cells and inflammatory factors, and the expression changes with the time of brain damage. IL-1β is expressed in the microglia at an early stage and it is expressed in astrocytes at the later stage. |
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