| 何 敏,杨 菁,蒲铃铃,刘 桦,尹小菲.小檗碱通过抑制经典的NLRP3炎症小体活化通路来下调LPS诱导的HUVEC炎症反应*[J].,2019,19(1):38-42 |
| 小檗碱通过抑制经典的NLRP3炎症小体活化通路来下调LPS诱导的HUVEC炎症反应* |
| Berberine Reduces Inflammation induced by Lipopolysaccharide through Suppressing Classical NLRP3 Inflammasome Activiation Pathwayin HUVEC* |
| 投稿时间：2018-03-06 修订日期：2018-03-28 |
| DOI：10.13241/j.cnki.pmb.2019.01.008 |
| 中文关键词: 小檗碱 脂多糖 HUVEC NLRP3炎症小体 |
| 英文关键词: Berberine Lipopolysaccharide HUVEC NLRP3 inflammasome |
| 基金项目:四川省大学生创新创业训练计划项目（201413705036） |
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| 中文摘要: |
| 摘要 目的：探讨小檗碱（Berberine, Ber）对脂多糖（Lipopolysaccharide, LPS）诱导的人脐静脉血管内皮细胞(Human Umbilical Vein Endothelial Cells, HUVEC)炎症反应的抑制作用及相关机制。方法：MTT法检测不同浓度的Ber对HUVEC存活率的影响。以0.05 μg/mL LPS刺激HUVEC建立炎症反应模型，并分别给予1.25、2.5、5μM Ber干预，Elisa检测Ber对炎症因子TNF-α、IL-1β分泌的影响，免疫印迹法检测Ber对NLRP3炎症小体信号通路中NLRP3、MyD88、IL-1β、TLR4、ASC和Caspase-1蛋白表达的影响。结果：不同浓度的Ber对HUVEC增殖均具有抑制作用，且基本呈现浓度梯度，IC50值接近5 μM；与LPS组相比，不同浓度Ber（1.25、2.5、5 μM）均可以显著下调HUVEC中TNF-α、IL-1β炎症因子的分泌（P<0.005及P<0.01），并可以显著抑制细胞中NLRP3、MyD88、IL-1β、TLR4、ASC和Caspase-1蛋白的表达（P<0.05），其抑制作用基本呈剂量关系，以5 μM Ber的抑制效果最佳。结论：Ber可以通过抑制经典的NLRP3炎症小体活化通路来下调LPS诱导的HUVEC炎症反应。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the inhibition and related molecular mechanisms of berberine on inflammation induced by LPS in HUVEC. Methods: The viability of different concentrations of berberine on HUVEC cells were determined by MTT. An inflammatory model of HUVEC was induced by 0.05 μg/mL LPS, then 1.25, 2.5, 5 μM berberine were used for intervention. The expressions of TNF-α, IL-1β in cell supernatants were evaluated by ELISA. The expressions of NLRP3, MyD88, IL-1β, TLR4, ASC and Caspase-1 proteins associating with Classical NLRP3 signaling pathway were evaluated by Western blot. Results: The proliferation of HUVEC were restrained by different concentrations of berberine, and there existed a concentration gradient . The IC50 value of berberine on HUVEC was close to 5μM. Compared with LPS group, different concentrations of berberine could significantly reduce the secretions of TNF-α, IL-1β and the expressions of NLRP3, MyD88, IL-1α, TLR4, ASC and Caspase-1 proteins in HUVEC. The inhibition had a dose dependent manner, and 5 μM berberine showed the greatest effect. Conclusion: Berberine could reduce inflammation induced by LPS in HUVEC through suppressing Classical NLRP3 inflammasome activiation pathway. |
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