| 张超峰,罗天航,钮宏文,邓 琳,祝 涛,方国恩.激活FXR抑制结肠癌细胞浸润转移及MMP-7的表达[J].,2018,(24):4633-4637 |
| 激活FXR抑制结肠癌细胞浸润转移及MMP-7的表达 |
| Activation of Farnesoid X Receptor Inhibited the Infiltration and Metastasis and MMP-7's Expression of Colon Cancer Cells |
| 投稿时间：2018-06-06 修订日期：2018-06-30 |
| DOI：10.13241/j.cnki.pmb.2018.24.006 |
| 中文关键词: 结肠癌 法尼酯X受体 肿瘤浸润转移 基质蛋白酶-7 |
| 英文关键词: Colon carcinoma Farnesoid X receptor Neoplasm invasiveness and metastasis Matrix metalloproteinase-7 |
| 基金项目:国家自然科学基金项目（81671886） |
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| 中文摘要: |
| 摘要 目的：探讨法尼酯X受体（FXR）特异性激动剂GW4064抑制结肠癌细胞浸润转移的机制。方法：在体外培养人结肠癌细胞HT-29，应用GW4064作用于结肠癌细胞，以四唑氮蓝还原法（MTT）检测细胞活性的变化。用transwell小室研究结肠癌细胞的迁移及浸润。用RT-PCR检测FXRmRNA及MMP-7mRNA表达的变化,用western blot检测FXR及MMP-7蛋白表达的变化。结果：MTT结果显示GW4064作用于人结直肠HT-29细胞的生长抑制率呈浓度依赖性；transwell小室结果显示GW4064抑制结肠癌细胞的浸润转移，与对照组相比，差异具有统计学意义（P<0.05），RT-PCR及Western blot显示GW4064促进FXR mRNA及蛋白表达，抑制MMP-7mRNA及蛋白的表达，与对照组相比差异有统计学意义（P<0.05）。结论：GW4064抑制结肠癌细胞的生长及转移，上调HT-29细胞FXR mRNA及蛋白的表达，下调HT-29细胞MMP-7 mRNA及蛋白的表达。FXR被激活后抑制结肠癌细胞转移，MMP-7可能是其作用通路之一。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the mechanism of the farnesoid X receptor (FXR) activation by GW4064 to inhibit the growth of colon cancer cells. Methods: The effects of specific FXR agonist (GW4064) on the growth of HT-29 cells were studied in vitro by MTT. The infiltration and metastasis of HT-29 cells were detected by the transwell inserts. The mRNA expression of FXR and MMP-7 were determined by RT-PCR, The protein expression of FXR and MMP-7 were measured by Western blot. Results: MTT results showed that the growth inhibition rate of GW4064 in human colorectal HT-29 cells was concentration dependent; Transwell inserts re- sults showed that GW4064 inhibited the invasion and metastasis of colon cancer cells. Compared with the control group, the difference was statistically significant (P<0.05). RT-PCR and Western blot display GW4064 promoted FXR mRNA and protein expression and inhi- bited the expression ofMMP-7. The expression of mRNA and protein was significantly different from that of the control group (P<0.05). Conclusion: GW4064 inhibited metastasis of colon cancer cells. The expression of mRNA and protein of FXR was up-regulated by FXR specific agonist in cell line HT-29, and MMP-7 is just opposite. The activation of FXR may inhibit the metastasis of colon cancer cells by inhibiting MMP-7. |
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