| 陈妍珂,张 徐,吴近珠,王婷婷,崔玉宝.hsa-miR-126的靶基因预测及其生物信息学分析[J].,2018,(23):4564-4570 |
| hsa-miR-126的靶基因预测及其生物信息学分析 |
| Prediction of Target Genes and Bioinformatics Analysis of Hsa-miR-126 |
| 投稿时间：2018-04-07 修订日期：2018-04-30 |
| DOI：10.13241/j.cnki.pmb.2018.23.039 |
| 中文关键词: hsa-miR-126 microRNA 靶基因 生物信息学 肿瘤 |
| 英文关键词: Hsa-miR-126 MicroRNA Target gene Bioinformatics Tumor |
| 基金项目:国家自然科学基金项目（NSFC31572319） |
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| 中文摘要: |
| 摘要 目的：采用生物信息学的方法预测hsa-miR-126的靶基因及相关功能，为后续研究提供理论基础。方法：采用PubMed搜索hsa-miR-126相关文章，总结目前研究进展，使用miRBase获得hsa-miR-126的基本信息，并用NCBI BLAST分析其序列保守性，以Targetscan 、miRanda及PicTar预测结果的两两交集为预测靶基因，并结合miRTarBase上已证实的靶基因作为基因集合做GO分析和Pathway分析。结果：搜索PubMed获得相关文献24篇，多数研究集中于hsa-miR-126与肿瘤的关系，hsa-miR-126在多种物种间具有高度保守性，其5个预测靶基因和46个已证实靶基因于白细胞迁移、对糖皮质激素反应、促进内皮细胞增殖、蛋白信号转导等有功能富集(P＜0.01)，于多种肿瘤如肾细胞癌、前列腺癌、非小细胞肺癌、胰腺癌有信号通路富集(P＜0.01)。结论：hsa-miR-126可能参与多种肿瘤的发生发展过程。 |
| 英文摘要: |
| ABSTRACT Objective: To predict the target genes and their functions of hsa-miR-126 via bioinformatics analysis, and provide theoretical basis for follow-up studies. Methods: Current studies of hsa-miR-126 were reviewed by PubMed. Basic information like sequence and gene characteristic were gathered from miRBase and confirmed by NCBI BLAST. The target genes of hsa-miR-126 were predicted through Targetscan, miRanda and PicTar. Combing with the confirmed target genes of hsa-miR-126 from miRTarBase, the functions of this set of target genes were analyzed via Gene Ontology analysis and pathway analysis. Results: Among the 24 pertinent lit- eratures, most studies focused on the relationships between hsa-miR-126 and multiple tumors. Hsa-miR-126 is highly conserved in differ- ent species. GO analysis showed that the 5 predicted＆46 confirmed target genes were enriched in biological processes like leukocyte mi- gration, response to glucocorticoid, positive regulation of endothelial cell proliferation and ras protein signal transduction(P<0.01), while pathway analysis indicated that the set of target genes were involved in renal cell carcinoma, prostate cancer, non-small cell lung cancer, pancreatic cancer and other pathways(P<0.01). Conclusion: Hsa-miR-126 may be involved in the pathogenesis of a variety of tumors. |
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